Kaufmann Anton, Dvorak Veronika, Crüzer Claudio, Butcher Patrick, Maden Kathryn, Walker Stephan, Widmer Mirjam, Schürmann Andreas
Official Food Control Authority, Fehrenstrasse 15, 8032 Zürich, Switzerland.
J AOAC Int. 2012 Mar-Apr;95(2):528-48. doi: 10.5740/jaoacint.11-074.
A validated LC/MS/MS-based multiresidue pesticide method was converted to an LC high-resolution MS single-stage Orbitrap platform. No changes regarding the cleanup and LC were made. Optimization of high-resolution MS-specific parameters and interface settings was kept at a minimum. The aim was to explore the capability of current Orbitrap technology to substitute for LC/MS/MS technology. The test included the quantitative performance (sensitivity, selectivity, linearity, accuracy, and precision) of some 240 analytes in three different matrixes. The LC/MS/MS instrumentation was operated at the edge of its technical limitations. A further extension of the number of analytes for LC/MS/MS would require the use of even narrower dwell times, significantly reducing sensitivity and reproducibility of measurement. No such limitations exist for the high-resolution technology. Similar performance was observed for both technologies. A current drawback of the high-resolution technology is the speed of data processing, which took significantly longer than for LC/MS/MS data due to the limited capabilities of the software.
一种经过验证的基于液相色谱-串联质谱的多残留农药方法被转换到液相色谱高分辨率质谱单级轨道阱平台。在净化和液相色谱方面未做任何改变。高分辨率质谱特定参数和接口设置的优化被保持在最低限度。目的是探索当前轨道阱技术替代液相色谱-串联质谱技术的能力。该测试包括在三种不同基质中约240种分析物的定量性能（灵敏度、选择性、线性、准确度和精密度）。液相色谱-串联质谱仪器在其技术极限边缘运行。进一步增加液相色谱-串联质谱分析物的数量将需要使用更短的驻留时间，这会显著降低测量的灵敏度和重现性。高分辨率技术不存在此类限制。两种技术观察到了相似的性能。高分辨率技术当前的一个缺点是数据处理速度，由于软件功能有限，其数据处理时间比液相色谱-串联质谱数据长得多。
