Wyss Institute for Biologically Inspired Engineering, Harvard University, Cambridge, Massachusetts 02138, USA.
ACS Nano. 2012 Jun 26;6(6):4796-805. doi: 10.1021/nn205073n. Epub 2012 Jun 6.
Poly(ethylene) glycol is commonly used to stabilize gold nanoparticles (GNPs). In this study, we evaluated the ability of cysteine-functionalized alginate-derived polymers to both provide colloidal stability to GNPs and avoid recognition and sequestration by the body's defense system. These polymers contain multiple reactive chemical groups (hydroxyl and carboxyl groups) that could allow for ready functionalization with, for example, cell-targeting ligands and therapeutic drugs. We report here that alginate-coupled GNPs demonstrate enhanced stability in comparison with bare citrate-coated GNPs and a similar lack of interaction with proteins in vitro and long in vivo circulation as PEG-coated GNPs.
聚乙二醇通常用于稳定金纳米颗粒（GNPs）。在这项研究中，我们评估了半胱氨酸功能化海藻酸盐衍生聚合物既能为 GNPs 提供胶体稳定性，又能避免被身体防御系统识别和隔离的能力。这些聚合物含有多个反应性化学基团（羟基和羧基），可以很容易地与例如细胞靶向配体和治疗性药物进行功能化。我们在这里报告，与裸露的柠檬酸盐涂层 GNPs 相比，海藻酸盐偶联的 GNPs 表现出增强的稳定性，并且在体外和体内长时间循环中与蛋白质的相互作用也类似于聚乙二醇涂层的 GNPs。
