Department of Gastroenterological Surgery, Osaka University Graduate School of Medicine, 2-2, E-2, Yamadaoka, Suita, Osaka 565-0871, Japan.
Surg Today. 2013 Feb;43(2):185-90. doi: 10.1007/s00595-012-0210-0. Epub 2012 Jun 1.
Collagen and fibrin are known to have potential use as a local drug-delivery system. This experimental study was designed to evaluate whether a fibrinogen-based collagen (FBC) fleece, coated with thrombin and aprotinin, can be used as an antibiotic delivery system.
In an in vitro study, gentamicin, fosfomycin, ampicillin, ciprofloxacin and dibekacin were absorbed by the FBC, Kirby-Bauer disks (KBDs), and expanded polytetrafluoroethylene. After washing with saline or phosphate buffer saline (PBS) 3 times for 6, 12 and 24 h, each sample was analyzed for antibiotic retention. In an in vivo study, we implanted the FBC onto mouse livers and dripped gentamicin and ciprofloxacin onto the FBC. The FBCs were subsequently collected and analyzed for their antibiotic activities.
After irrigation with saline, each antibiotic showed different activities. After PBS washing, the FBC impregnated with each antibiotic had higher activity than the KBDs, and inhibited the bacterial growth by 60-80 % compared to the control. Gentamicin dripped onto the FBC could inhibit bacterial growth after 48 h in vivo without affecting the hemostatic properties of the FBC. However, the FBC treated with ciprofloxacin exhibited antibacterial activity for only 3 h.
Some bases, including FBC, can retain antibacterial activities dependent on the ingredients of the base and the type of antibiotic. Gentamicin, but not ciprofloxacin, was retained in the FBC in vivo. These results suggest that absorbent FBC might be useful not only as hemostatic material, but also as a local drug-delivery system.
胶原蛋白和纤维蛋白已被证明具有作为局部药物递送系统的潜力。本实验研究旨在评估基于纤维蛋白原的胶原蛋白（FBC）纤维是否可以涂覆凝血酶和抑肽酶，作为抗生素递送系统。
在体外研究中，庆大霉素、磷霉素、氨苄西林、环丙沙星和地贝卡星被 FBC、Kirby-Bauer 圆盘（KBD）和膨胀聚四氟乙烯吸收。用盐水或磷酸盐缓冲盐水（PBS）洗涤 3 次，每次 6、12 和 24 h 后，分析每个样品的抗生素保留情况。在体内研究中，我们将 FBC 植入小鼠肝脏，并将庆大霉素和环丙沙星滴在 FBC 上。随后收集 FBC 并分析其抗生素活性。
用盐水冲洗后，每种抗生素表现出不同的活性。用 PBS 洗涤后，与 KBD 相比，每种抗生素浸渍的 FBC 具有更高的活性，与对照组相比，抑制细菌生长 60-80%。滴在 FBC 上的庆大霉素在体内 48 小时后可以抑制细菌生长，而不会影响 FBC 的止血性能。然而，用环丙沙星处理的 FBC 仅表现出 3 小时的抗菌活性。
某些基质，包括 FBC，可以保留依赖于基质成分和抗生素类型的抗菌活性。在体内，庆大霉素而非环丙沙星被保留在 FBC 中。这些结果表明，吸收性 FBC 不仅可用作止血材料，还可用作局部药物递送系统。
