National Engineering Laboratory for Efficient Utilization of Soil and Fertilizer Resources, Key Laboratory of the Agricultural Environment of the Universities of Shandong, College of Resources and Environment, Shandong Agriculture University, Taian, People's Republic of China.
Environ Toxicol Chem. 2012 Sep;31(9):2035-40. doi: 10.1002/etc.1907. Epub 2012 Jul 11.
To assess the combined toxic effects of atrazine and cadmium on earthworms, specimens of Eisenia fetida were exposed in artificial soil to three concentrations of atrazine (0, 0.5, and 2.5 mg kg(-1)) and a range of concentrations of cadmium (Cd; 0, 0.03, 0.3, and 3.0 mg kg(-1)) both singly and as mixtures. The DNA damage and internal atrazine and cadmium concentrations were assessed in earthworms on days 7, 14, 21, and 28 of the treatment. The results showed that the olive tail moments (OTMs) at individual atrazine and cadmium concentrations were significantly higher than those of the controls (p < 0.01). As exposure to atrazine or cadmium progressed, the OTMs increased and the maximum value occurred on day 28. In all combined treatments, the OTMs were much less than those of the sum of individual atrazine and cadmium OTMs, suggesting that the combined effects of atrazine and cadmium were less than additive. The less than additive toxicity of atrazine and cadmium might be due to the formation of atrazine-cadmium complexes or the activation of detoxification isozymes. Moreover, there was a significant correlation between internal atrazine or cadmium concentrations and DNA damage in most exposures, indicating that body residues were consistent with toxicity response.
为了评估莠去津和镉对蚯蚓的联合毒性效应，将赤子爱胜蚓暴露在人工土壤中，分别接触三种莠去津浓度（0、0.5 和 2.5mg/kg）和一系列镉浓度（0、0.03、0.3 和 3.0mg/kg），这些浓度既可以单独使用，也可以混合使用。在处理的第 7、14、21 和 28 天，评估了蚯蚓的 DNA 损伤以及体内莠去津和镉的浓度。结果表明，在个体莠去津和镉浓度下，橄榄尾矩（OTM）明显高于对照组（p<0.01）。随着接触莠去津或镉的进行，OTM 增加，最大值出现在第 28 天。在所有联合处理中，OTM 远小于个体莠去津和镉 OTM 的总和，表明莠去津和镉的联合效应小于相加效应。莠去津和镉的毒性小于相加效应可能是由于莠去津-镉复合物的形成或解毒同工酶的激活。此外，在大多数暴露中，体内莠去津或镉浓度与 DNA 损伤之间存在显著相关性，表明体内残留与毒性反应一致。
