用于灰飞虱（半翅目：飞虱科）的九个多态性微卫星的开发与特征分析
Development and characterization of nine polymorphic microsatellites for the small brown planthopper Laodelphax striatellus (Hemiptera: Delphacidae).
作者信息
Sun J-T, Li J-B, Yang X-M, Hong X-Y
机构信息
Department of Entomology, Nanjing Agricultural University, Nanjing, Jiangsu, China.
出版信息
Genet Mol Res. 2012 May 21;11(2):1526-31. doi: 10.4238/2012.May.21.9.
Nine microsatellite loci were isolated from the genome of Laodelphax striatellus (Homoptera: Delphacidae) by constructing (TC)(6)(AC)(5) and (AG)(6)(AC)(5) compound SSR-enriched libraries using suppression-PCR procedures. These loci were found to be highly polymorphic, with 13 to 30 alleles per locus in the three populations that we investigated (Jiangsu, Shandong and Zhejiang). The observed and expected heterozygosities ranged from 0.255 to 0.833 and 0.392 to 0.929, respectively. These microsatellite markers can be used for the study of population genetic structure and genetic diversity of L. striatellus.
通过使用抑制性聚合酶链反应构建(TC)(6)(AC)(5)和(AG)(6)(AC)(5)复合SSR富集文库，从灰飞虱（同翅目：飞虱科）基因组中分离出9个微卫星位点。在所调查的三个种群（江苏、山东和浙江）中，这些位点具有高度多态性，每个位点有13至30个等位基因。观察到的杂合度和预期杂合度分别为0.255至0.833和0.392至0.929。这些微卫星标记可用于研究灰飞虱的种群遗传结构和遗传多样性。
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