来自两个富集基因组文库的多态性微卫星位点用于黄斑鱼（Miichthys miiuy，石首鱼科）的遗传分析。

Polymorphic microsatellite loci from two enriched genomic libraries for the genetic analysis of the miiuy croaker, Miichthys miiuy (Sciaenidae).

作者信息

Wang R X, Xu T J, Sun Y N, He G Y

机构信息

China-UK HUST-RRes Genetic Engineering and Genomics Joint Laboratory, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, P.R. China.

出版信息

Genet Mol Res. 2010 May 18;9(2):931-4. doi: 10.4238/vol9-2gmr806.

DOI:10.4238/vol9-2gmr806

PMID:20486088

Abstract

Twelve polymorphic microsatellites from the (AG)(13) and (CA)(13) enriched genomic libraries of Miichthys miiuy were isolated and characterized in a test population; the number of alleles ranged from two to nine. The observed and expected heterozygosities ranged from 0.1923 to 1.0000 and from 0.2633 to 0.8337, respectively. Three loci deviated from Hardy-Weinberg equilibrium, and linkage disequilibrium between five pairs of loci was significant. These polymorphic microsatellite loci can be used for genetic diversity analysis and molecular-assisted breeding of M. miiuy.

摘要

从鮸鱼的(AG)(13)和(CA)(13)富集基因组文库中分离出12个多态性微卫星，并在一个测试群体中进行了特征分析；等位基因数量从2个到9个不等。观察到的杂合度和期望杂合度分别在0.1923至1.0000和0.2633至0.8337之间。三个位点偏离哈迪-温伯格平衡，五对位点之间的连锁不平衡显著。这些多态性微卫星位点可用于鮸鱼的遗传多样性分析和分子辅助育种。