Laboratory of Physical Biology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China.
Nanoscale. 2012 Jul 7;4(13):3861-6. doi: 10.1039/c2nr30407d. Epub 2012 Jun 1.
Graphene oxide (GO), has created an unprecedented opportunity for development and application in biology, due to its abundant functional groups and well water solubility. Recently, the potential toxicity of GO in the environment and in humans has garnered more and more attention. In this paper, we systematically studied the cytotoxicity of GO nanosheets via examining the effect of GO on the morphology, viability and differentiation of a human neuroblastoma SH-SY5Y cell line, which was an ideal model used to study neuronal disease in vitro. The results suggested that GO had no obvious cytotoxicity at low concentration (<80 μg mL(-1)) for 96 h, but the viability of cells exhibited dose- and time-dependent decreases at high concentration (≥ 80 μg mL(-1)). Moreover, GO did not induce apoptosis. Very interestingly, GO significantly enhanced the differentiation of SH-SY5Y induced-retinoic acid (RA) by evaluating neurite length and the expression of neuronal marker MAP2. These data provide a promising application for neurodegenerative diseases.
氧化石墨烯（GO）由于其丰富的官能团和良好的水溶性，为其在生物学中的发展和应用创造了前所未有的机会。最近，GO 在环境和人类中的潜在毒性引起了越来越多的关注。在本文中，我们通过研究 GO 对人神经母细胞瘤 SH-SY5Y 细胞系形态、活力和分化的影响，系统地研究了 GO 纳米片的细胞毒性，该细胞系是体外研究神经元疾病的理想模型。结果表明，GO 在 96 h 内低浓度（<80μg/mL）时无明显细胞毒性，但高浓度（≥80μg/mL）时细胞活力呈剂量和时间依赖性下降。此外，GO 不会诱导细胞凋亡。有趣的是，通过评估神经突长度和神经元标志物 MAP2 的表达，GO 显著增强了诱导型维甲酸（RA）诱导的 SH-SY5Y 分化。这些数据为神经退行性疾病的治疗提供了有希望的应用。
