胆汁酸对大鼠胃蛋白酶原改变的幽门腺发育的影响。

Effects of bile acids on development of pepsinogen-altered pyloric glands in rats.

作者信息

Yamaguchi S, Tatematsu M, Furihata C, Takaba K, Ito N

机构信息

First Department of Pathology, Nagoya City University Medical School, Japan.

出版信息

Cancer Lett. 1990 Dec 3;55(2):129-34. doi: 10.1016/0304-3835(90)90022-p.

DOI:10.1016/0304-3835(90)90022-p

PMID:2265411

Abstract

The effects of dietary bile acids on the development of pepsinogenaltered pyloric glands (PAPG) were examined. Male WKY/NCrj rats were given a single dose of 160 mg/kg body wt. of N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) by gastric intubation and fed basal diet containing 0.3% sodium taurocholate (Na-TC), 0.3% sodium cholate (Na-C), 0.3% sodium glycocholate (Na-GC), 0.3% sodium tauroglycocholate, 0.3% sodium deoxycholate, 0.1% chenodeoxycholic acid or 0.5% lithocholic acid for 14 weeks. All rats also received 1 ml of saturated NaCl solution 4 times by i.g. intubation. At the end of week 16, the animals were killed, and the number of PAPG per cm of mucosal length was determined immunohistochemically. Na-TC, Na-C and Na-GC significantly increased the number of PAPG over the control value, suggesting that they may have activity to promote gastric carcinogenesis.

摘要

研究了膳食胆汁酸对胃蛋白酶原改变的幽门腺（PAPG）发育的影响。通过胃内插管给雄性WKY/NCrj大鼠单次注射160mg/kg体重的N-甲基-N'-硝基-N-亚硝基胍（MNNG），并喂食含0.3%牛磺胆酸钠（Na-TC）、0.3%胆酸钠（Na-C）、0.3%甘氨胆酸钠（Na-GC）、0.3%牛磺甘胆酸钠、0.3%脱氧胆酸钠、0.1%鹅去氧胆酸或0.5%石胆酸的基础饮食14周。所有大鼠还通过胃内插管接受4次1ml饱和NaCl溶液。在第16周结束时，处死动物，通过免疫组织化学测定每厘米黏膜长度的PAPG数量。Na-TC、Na-C和Na-GC使PAPG数量显著高于对照值，表明它们可能具有促进胃癌发生的活性。