Tatematsu M, Aoki T, Asamoto M, Furihata C, Ito N
Jpn J Cancer Res. 1987 Apr;78(4):312-6.
Sequential quantitative analyses were made of pepsinogen 1 (Pg 1) decreased pyloric glands after treating male WKY rats first with N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) and then with N-ethyl-N'-nitro-N-nitrosoguanidine (ENNG) as a second gastric carcinogen or sodium taurocholate (Na-TC) as a gastric promoter. Animals received a single dose of MNNG (160 mg/kg body weight) by gastric intubation followed two weeks later by either ENNG in drinking water (100 micrograms/ml) (group 1), basal diet containing 0.25% Na-TC (group 2), or basal diet and tap water (group 3), from weeks 3 to 24. Animals were sacrificed at weeks 8, 12, 16, 20 and 24. Sections of the pyloric mucosa were investigated for Pg 1 immunostaining. In comparison with group 3, induction of Pg 1 decreased pyloric glands was significantly enhanced by ENNG from week 8 and by Na-TC from week 16. The former exerted a significantly stronger effect at each time point. The results suggest that Pg 1 decreased pyloric glands represent a good marker for early detection of gastric carcinogens and promoters in in vivo test systems.
对雄性WKY大鼠先给予N-甲基-N'-硝基-N-亚硝基胍（MNNG），然后再给予作为第二种胃癌致癌物的N-乙基-N'-硝基-N-亚硝基胍（ENNG）或作为胃促癌剂的牛磺胆酸钠（Na-TC）处理后，对胃蛋白酶原1（Pg 1）减少的幽门腺进行了序贯定量分析。动物经胃插管接受单次剂量的MNNG（160 mg/kg体重），两周后，从第3周到第24周，第1组动物饮用含100微克/毫升ENNG的水，第2组动物食用含0.25% Na-TC的基础饲料，第3组动物食用基础饲料并饮用自来水。在第8、12、16、20和24周处死动物。对幽门黏膜切片进行Pg 1免疫染色研究。与第3组相比，从第8周起ENNG和从第16周起Na-TC均显著增强了Pg 1减少的幽门腺的诱导。在每个时间点，前者的作用明显更强。结果表明，Pg 1减少的幽门腺是体内检测系统中早期检测胃癌致癌物和促癌剂的良好标志物。
