检测真核翻译起始因子 4E 及其在肝癌中的临床意义。
Detection of eukaryotic translation initiation factor 4E and its clinical significance in hepatocellular carcinoma.
机构信息
Gastroenterology Department, The Second Military Medical University, Changzheng Hospital, Shanghai 200003, China.
出版信息
World J Gastroenterol. 2012 May 28;18(20):2540-4. doi: 10.3748/wjg.v18.i20.2540.
AIM
To study the expression of eukaryotic translation initiation factor 4E (eIF4E), which is closely correlated with malignant tumors, and its relationship to prognosis in hepatocellular carcinoma.
METHODS
Western blotting was performed to quantify the elF4E protein expression in the normal human liver cell line L02 and the hepatoma cell lines Hep3B, HepG2, and Huh7. Forty-six hepatocellular carcinoma samples with complete clinical data were obtained from Changzheng Hospital during the period of December 2008 to July 2009. The expression of eIF4E in the tumor samples and their adjacent tissues were detected by immunohistochemistry. The relationship between the test results and hepatocellular carcinoma (HCC) prognosis was statistically analysed by using a COX proportional hazard model.
RESULTS
Western blotting analysis showed that there were distinct eIF4E protein bands in all three of the hepatoma cell lines. In particular, the HepG2 cell line had the highest level of eIF4E protein expression. The L02 cell group had a low eIF4E expression. Immunohistochemical assay showed that there were 32 cases in which the tumour tissue expression was higher than their adjacent tissues, accounting for 69.57%. There were also 14 cases in which the tumour tissue expression was lower or no significant difference was found, accounting for 30.43%. COX proportional hazards model analysis showed that HCC prognosis was related to the depth of invasion, the overexpression of eIF4E and p53, possibly as independent HCC prognostic predictors.
CONCLUSION
In summary, eIF4E expression is associated with liver cancer, and patients with high eIF4E expression levels have a higher risk of recurrence.
目的
研究与恶性肿瘤密切相关的真核翻译起始因子 4E（eIF4E）的表达及其与肝癌预后的关系。
方法
采用 Western blot 法检测正常肝细胞系 L02 和肝癌细胞系 Hep3B、HepG2、Huh7 中 eIF4E 蛋白的表达。收集 2008 年 12 月至 2009 年 7 月长征医院 46 例完整临床资料的肝癌组织标本，采用免疫组化法检测肿瘤组织及其邻近组织中 eIF4E 的表达，采用 COX 比例风险模型对检验结果与肝癌（HCC）预后的关系进行统计学分析。
结果
Western blot 分析显示，三种肝癌细胞系均有明显的 eIF4E 蛋白条带，其中 HepG2 细胞系 eIF4E 蛋白表达水平最高，L02 细胞系 eIF4E 蛋白表达水平较低。免疫组化检测显示，肿瘤组织表达高于邻近组织 32 例，占 69.57%；肿瘤组织表达低于或无明显差异 14 例，占 30.43%。COX 比例风险模型分析显示，肝癌预后与肿瘤浸润深度、eIF4E 和 p53 过表达有关，可能是独立的 HCC 预后预测因子。
结论
综上所述，eIF4E 的表达与肝癌有关，eIF4E 高表达的患者复发风险更高。
Zotero 插件