College of Plant Science, Jilin University, Changchun 130062, China.
College of Plant Health and Medicine, Qingdao Agricultural University, Qingdao 266109, China.
Int J Mol Sci. 2018 Sep 27;19(10):2946. doi: 10.3390/ijms19102946.
(Lib.) de Bary is a devastating necrotrophic fungal pathogen attacking a broad range of agricultural crops. In this study, although the transcript accumulation of SsNsd1, a GATA-type IVb transcription factor, was much lower during the vegetative hyphae stage, its mutants completely abolished the development of compound appressoria. To further elucidate how SsNsd1 influenced the appressorium formation, we conducted proteomics-based analysis of the wild-type and Δ mutant by two-dimensional electrophoresis (2-DE). A total number of 43 differentially expressed proteins (≥3-fold change) were observed. Of them, 77% were downregulated, whereas 14% were upregulated. Four protein spots fully disappeared in the mutants. Further, we evaluated these protein sequences by mass spectrometry analysis of the peptide mass and obtained functionally annotated 40 proteins, among which only 17 proteins (38%) were identified to have known functions including energy production, metabolism, protein fate, stress response, cellular organization, and cell growth and division. However, the remaining 23 proteins (56%) were characterized as hypothetical proteins among which four proteins (17%) were predicted to contain the signal peptides. In conclusion, the differentially expressed proteins identified in this study shed light on the Δ mutant-mediated appressorium deficiency and can be used in future investigations to better understand the signaling mechanisms of SsNsd1 in .
（Lib.）de Bary 是一种具有破坏性的坏死性真菌病原体，可攻击广泛的农业作物。在这项研究中，尽管 GATA 型 IVb 转录因子 SsNsd1 的转录本在营养菌丝阶段的积累量较低，但它的突变体完全取消了复合附着胞的发育。为了进一步阐明 SsNsd1 如何影响附着胞的形成，我们通过二维电泳（2-DE）对野生型和Δ突变体进行了基于蛋白质组学的分析。观察到总共有 43 个差异表达的蛋白质（≥3 倍变化）。其中，77%下调，14%上调。在突变体中，四个蛋白质斑点完全消失。此外，我们通过肽质量的质谱分析评估了这些蛋白质序列，并获得了功能注释的 40 个蛋白质，其中只有 17 个蛋白质（38%）被鉴定为具有已知功能，包括能量产生、代谢、蛋白质命运、应激反应、细胞组织和细胞生长和分裂。然而，其余 23 个蛋白质（56%）被特征化为假定蛋白质，其中 4 个蛋白质（17%）被预测含有信号肽。总之，本研究中鉴定的差异表达蛋白阐明了Δ突变体介导的附着胞缺陷，并可用于未来的研究，以更好地理解 SsNsd1 中的信号转导机制。
