Department of Virology, Erasmus Medical Center, Rotterdam, The Netherlands.
PLoS Pathog. 2012;8(5):e1002682. doi: 10.1371/journal.ppat.1002682. Epub 2012 May 24.
A fatal human case of Duvenhage virus (DUVV) infection in a Dutch traveller who had returned from Kenya was reported in 2007. She exhibited classical symptoms of rabies encephalitis with distinct pathological findings. In the present study we describe the isolation and characterization of DUVV in vitro and its passage in BALB/c mice. The virus proved to be neuroinvasive in both juvenile and adult mice, resulting in about 50% lethality upon peripheral infection. Clinical signs in infected mice were those of classical rabies. However, the distribution of viral antigen expression in the brain differed from that of classical rabies virus infection and neither inclusion bodies nor neuronal necrosis were observed. This is the first study to describe the in vitro and in vivo isolation and characterization of DUVV.
2007 年，一名从肯尼亚返回的荷兰旅行者感染杜文汗病毒（Duvenhage virus，DUVV）后死亡，这是首例致命的人类病例报告。她表现出典型的狂犬病脑炎症状，并伴有明显的病理发现。在本研究中，我们描述了 DUVV 的体外分离和鉴定及其在 BALB/c 小鼠中的传代。该病毒在幼年和成年小鼠中均具有神经侵袭性，导致外周感染后约 50%的致死率。感染小鼠的临床症状为典型的狂犬病。然而，病毒抗原在大脑中的表达分布与经典狂犬病病毒感染不同，未观察到包涵体和神经元坏死。这是首次描述 DUVV 的体外和体内分离与鉴定的研究。
