Nilsson R, Lindgren L, Lilliehorn P
Department of Immunology, Excorim, Lund, Sweden.
Clin Exp Immunol. 1990 Dec;82(3):440-4. doi: 10.1111/j.1365-2249.1990.tb05468.x.
A technique for extracorporeal immunoadsorption of circulating specific antibodies from the plasma of rats is described. Catheterization of rats was performed using the carotid and the jugular blood vessels. The rats were treated non-anaesthetized. Blood was pumped continuously through a hollow-fibre plasma filter at a rate of 1.5 ml/min and plasma was separated and passed through an adsorbent column at a flow of 0.2 ml/min. The treated plasma was mixed with the blood cells before being returned to the rat. The column contained the antigen covalently linked to agarose beads. About three plasma volumes were treated during a period of 3 h and 30 min, which resulted in an antibody depletion of about 90-95%. The antibody levels returned to pre-adsorption levels after 4-5 days, or in some cases even exceeded the titres before treatment.
描述了一种从大鼠血浆中体外免疫吸附循环特异性抗体的技术。使用颈动脉和颈静脉对大鼠进行插管。大鼠在未麻醉状态下接受治疗。血液以1.5毫升/分钟的流速持续泵入微孔纤维血浆过滤器，血浆被分离出来并以0.2毫升/分钟的流速通过吸附柱。经过处理的血浆在返回大鼠体内之前与血细胞混合。该柱含有与琼脂糖珠共价连接的抗原。在3小时30分钟的时间段内，约三个血浆体积被处理，这导致抗体消耗约90%至95%。抗体水平在4至5天后恢复到吸附前水平，或者在某些情况下甚至超过治疗前的滴度。
