Ryan C J, Pusey C D, Aslam M, Gaylor J D, Maini R, Courtney J M
Artif Organs. 1986 Apr;10(2):135-44. doi: 10.1111/j.1525-1594.1986.tb02532.x.
Repeated membrane plasma separation with on-line sorbent treatment of plasma was performed in unrestrained and conscious rats, thus avoiding the possible effects of repeated stress and exposure to anesthetic agents. The procedure was well tolerated, even after four consecutive perfusions. Blood flow was 0.7 ml/min, with a transmembrane pressure of 18.6 mm Hg and plasma filtration rate of 0.21 ml/min. This allowed 12 ml of plasma (greater than 1 plasma volume) to be treated within 1 h. Levels of albumin, immunoglobulin G, and C3 remained constant during each perfusion and were not significantly different from those of animals subjected to control procedures. Results obtained from filtrate were comparable at 15 and 60 min with values obtained from whole blood, with sieving coefficients of approximately 1. Leukocyte, erythrocyte, and thrombocyte counts remained unchanged during each plasma perfusion. A progressive rise in leukocyte counts occurred following successive perfusions, but this was true also of animals subjected to control procedures.
在未束缚且清醒的大鼠中进行了重复的膜血浆分离并对血浆进行在线吸附剂处理，从而避免了重复应激和接触麻醉剂可能产生的影响。即使在连续四次灌注后，该操作也具有良好的耐受性。血流速度为0.7 ml/分钟，跨膜压力为18.6毫米汞柱，血浆滤过率为0.21 ml/分钟。这使得在1小时内能够处理12毫升血浆（大于1个血浆容量）。在每次灌注过程中，白蛋白、免疫球蛋白G和C3的水平保持恒定，且与接受对照程序的动物的水平无显著差异。在15分钟和60分钟时从滤液中获得的结果与从全血中获得的值相当，筛分系数约为1。在每次血浆灌注过程中，白细胞、红细胞和血小板计数保持不变。连续灌注后白细胞计数逐渐上升，但接受对照程序的动物也是如此。
