Krieter H, Brückner U B, Krumwieh D, Seiler F R, Messmer K
Department of Experimental Surgery, University of Heidelberg, FRG.
Eur Surg Res. 1990;22(3):121-7. doi: 10.1159/000129092.
Six splenectomized beagles of either sex (13.8 +/- 2.2 kg) were randomly treated either with 500 U/kg recombinant human erythropoietin (rhu-EPO) (verum group, n = 3) or an equivalent volume of the vehicle (placebo group, n = 3). Both solutions were given intravenously for 3 days. At day 4 after onset of treatment, the dogs were anesthetized and subjected to isovolemic hemodilution using 6% Dextran 60 (MW 60,000) down to a hematocrit of 0.10. During the recovery period vehicle or rhu-EPO was given every other day until the hematocrit reached control values. Every day venous blood samples were withdrawn, and the hematocrit as well as the concentrations of hemoglobin and 2,3-diphosphoglycerate were determined. In addition, the platelets and reticulocytes were counted. Treatment with rhu-EPO shortened the time of hematocrit recovery from 20 (placebo) to 11 days (p less than 0.05). The reticulocyte count peaked at day 2 (verum) versus day 5 (placebo). These findings indicate a successful stimulation of red blood cell production after extreme hemodilution in animals treated with erythropoietin. Therefore, rhu-EPO may allow to optimize blood donation programs as well as preoperative hemodilution and yield both, higher amounts of autologous blood and an accelerated reversal of dilutional anemia.
选用6只体重13.8±2.2kg的已切除脾脏的比格犬，雌雄不限，随机分为两组，分别给予500U/kg重组人促红细胞生成素（rhu-EPO）（实验组，n = 3）或等量的赋形剂（安慰剂组，n = 3）。两种溶液均静脉注射，持续3天。在开始治疗后的第4天，对犬进行麻醉，使用6%的右旋糖酐60（分子量60,000）进行等容血液稀释，使血细胞比容降至0.10。在恢复期间，每隔一天给予赋形剂或rhu-EPO，直至血细胞比容达到对照值。每天采集静脉血样，测定血细胞比容、血红蛋白浓度和2,3-二磷酸甘油酸浓度。此外，还对血小板和网织红细胞进行计数。rhu-EPO治疗使血细胞比容恢复时间从20天（安慰剂组）缩短至11天（p < 0.05）。网织红细胞计数在第2天（实验组）达到峰值，而安慰剂组在第5天达到峰值。这些发现表明，在用促红细胞生成素治疗的动物中，极端血液稀释后红细胞生成受到成功刺激。因此，rhu-EPO可能有助于优化献血计划以及术前血液稀释，从而获得更多的自体血，并加速稀释性贫血的逆转。
