78 kDa葡萄糖调节蛋白的可逆ADP核糖基化作用

Reversible ADP-ribosylation of the 78 kDa glucose-regulated protein.

作者信息

Leno G H, Ledford B E

机构信息

Molecular and Cellular Biology and Pathobiology Program, Medical University of South Carolina, Charleston 29425.

出版信息

FEBS Lett. 1990 Dec 10;276(1-2):29-33. doi: 10.1016/0014-5793(90)80499-9.

DOI:10.1016/0014-5793(90)80499-9

PMID:2265706

Abstract

Starvation of Mouse hepatoma cells for essential amino acids or glucose results in the mono-ADP-ribosylation of the 78 kDa glucose-regulated protein, GRP78. Here we show that the ADP-ribosylated and non-ADP-ribosylated forms of GRP78 are interconvertible during tryptophan starvation and refeeding. In addition, the ADP-ribosylation of GRP78 was shown to be reversible even during nutritional stress. The overexpressed pool of non-ADP-ribosylated GRP78 synthesized during tunicamycin treatment was available for ADP-ribosylation during subsequent amino acid starvation, especially in the absence of tunicamycin. The reversible ADP-ribosylation of GRP78 could be part of a metabolic control mechanism in operation during nutritional stress.

摘要

小鼠肝癌细胞缺乏必需氨基酸或葡萄糖会导致78kDa葡萄糖调节蛋白GRP78出现单ADP核糖基化。我们在此表明，在色氨酸饥饿和重新喂食过程中，GRP78的ADP核糖基化形式和非ADP核糖基化形式是可以相互转换的。此外，即使在营养应激期间，GRP78的ADP核糖基化也被证明是可逆的。在衣霉素处理期间合成的非ADP核糖基化GRP78的过表达库，在随后的氨基酸饥饿期间可用于ADP核糖基化，尤其是在没有衣霉素的情况下。GRP78的可逆ADP核糖基化可能是营养应激期间运行的代谢控制机制的一部分。