Gramstat A, Courtpozanis A, Rohde W
Max-Planck-Institut für Züchtungsforschung, Köln, FRG.
FEBS Lett. 1990 Dec 10;276(1-2):34-8. doi: 10.1016/0014-5793(90)80500-i.
The 3' terminal 1.4 kb segment of potato virus M (PVM) genomic RNA was cloned and sequenced. This part of the viral genome encodes the capsid protein CP as well as a 12 kDa protein of as yet unknown function. Both proteins were expressed in bacteria and their nucleic acid-binding properties studied. The 12 kDa protein (pr12), but not the capsid protein bound single- and double-stranded nucleic acids. This property of pr12 in conjunction with a zinc finger motif located adjacent to a basic region of the 12 kDa protein suggests that it may act as a regulatory factor during virus replication.
克隆并测序了马铃薯M病毒（PVM）基因组RNA的3'末端1.4 kb片段。病毒基因组的这一部分编码衣壳蛋白CP以及一种功能未知的12 kDa蛋白。这两种蛋白均在细菌中表达，并研究了它们的核酸结合特性。12 kDa蛋白（pr12）能结合单链和双链核酸，而衣壳蛋白不能。pr12的这一特性，再加上位于12 kDa蛋白碱性区域附近的锌指基序，表明它可能在病毒复制过程中作为一种调节因子发挥作用。
