van der Wilk F, Huisman M J, Cornelissen B J, Huttinga H, Goldbach R
Agricultural University Wageningen, Department of Virology, The Netherlands.
FEBS Lett. 1989 Mar 13;245(1-2):51-6. doi: 10.1016/0014-5793(89)80190-5.
The nucleotide sequence of the genomic RNA of potato leafroll virus was determined and its genetic organization deduced. The RNA is 5882 nucleotides long and contains 6 open reading frames (ORFs) encoding proteins of 70, 70, 56, 28, 23 and 17 kDa. The putative genes for the coat protein (23 kDa) and the RNA-dependent RNA polymerase (70 kDa) were identified by interviral amino acid sequence homologies. For expression of the different ORFs, translational frameshift and readthrough events are proposed.
测定了马铃薯卷叶病毒基因组RNA的核苷酸序列，并推导了其基因结构。该RNA长5882个核苷酸，包含6个开放阅读框（ORF），编码70、70、56、28、23和17 kDa的蛋白质。通过病毒间氨基酸序列同源性鉴定了外壳蛋白（23 kDa）和RNA依赖的RNA聚合酶（70 kDa）的推定基因。对于不同ORF的表达，提出了翻译移码和通读事件。
