Thekke-Veetil Thanuja, McCoppin Nancy K, Hobbs Houston A, Hartman Glen L, Lambert Kris N, Lim Hyoun-Sub, Domier Leslie L
Department of Crop Sciences, University of Illinois, Urbana, IL 61801, USA.
Soybean/Maize Germplasm, Pathology, and Genetics Research Unit, United States Department of Agriculture-Agricultural Research Service, Urbana, IL 61801, USA.
Pathogens. 2021 Feb 18;10(2):223. doi: 10.3390/pathogens10020223.
A novel member of the genus, provisionally named soybean carlavirus 1 (SCV1), was discovered by RNA-seq analysis of randomly collected soybean leaves in Illinois, USA. The SCV1 genome contains six open reading frames that encode a viral replicase, triple gene block proteins, a coat protein (CP) and a nucleic acid binding protein. The proteins showed highest amino acid sequence identities with the corresponding proteins of red clover carlavirus A (RCCVA). The predicted amino acid sequence of the SCV1 replicase was only 60.6% identical with the replicase of RCCVA, which is below the demarcation criteria for a new species in the family . The predicted replicase and CP amino acid sequences of four SCV1 isolates grouped phylogenetically with those of members of the genus in the family . The features of the encoded proteins, low nucleotide and amino acid sequence identities of the replicase with the closest member, and the phylogenetic grouping suggest SCV1 is a new member of the genus.
通过对美国伊利诺伊州随机采集的大豆叶片进行RNA测序分析，发现了该属的一个新成员，暂命名为大豆香石竹潜隐病毒1（SCV1）。SCV1基因组包含六个开放阅读框，编码一种病毒复制酶、三基因块蛋白、一种外壳蛋白（CP）和一种核酸结合蛋白。这些蛋白与红三叶草香石竹潜隐病毒A（RCCVA）的相应蛋白具有最高的氨基酸序列同一性。SCV1复制酶的预测氨基酸序列与RCCVA的复制酶只有60.6%的同一性，低于该科新物种的划分标准。四个SCV1分离株的预测复制酶和CP氨基酸序列在系统发育上与该科该属成员的序列归为一类。编码蛋白的特征、复制酶与最接近成员的低核苷酸和氨基酸序列同一性以及系统发育分组表明SCV1是该属的一个新成员。
