Institute of Pharmacy/Pharmacognosy, CCB - Center of Chemistry and Biomedicine, Leopold Franzens University Innsbruck, Innsbruck, Austria.
J Chromatogr A. 2012 Oct 12;1259:50-61. doi: 10.1016/j.chroma.2012.05.032. Epub 2012 May 12.
Combining the most powerful separation techniques, i.e. liquid chromatography (LC) or capillary electrophoresis (CE) with a information rich detection system - the mass spectrometer or the nuclear magnetic resonance (NMR) spectrometer - has been pursued for more than three decades. This compilation shall provide an overview of the advantages and limitations of the LC-NMR hyphenation in the light of its most valued application-the unequivocal analyte identification. Especially the post LC trapping of analytes with an in-line solid phase extraction (SPE) device prior to transferring the analyte of interest to the NMR spectrometer (LC-SPE-NMR) proved to be a robust installation allowing a significant cut-down of the amount of analyte needed for the generation of high quality heteronuclear NMR shift correlation data. Different available technical realizations will be discussed and typical application examples from natural product research and from industrial settings will be given.
将最强大的分离技术，如液相色谱（LC）或毛细管电泳（CE）与信息丰富的检测系统——质谱仪或核磁共振（NMR）光谱仪相结合，已经被研究了三十多年。本综述旨在概述 LC-NMR 联用在分析物的明确鉴定方面的最有价值的应用中的优势和局限性。特别是在将感兴趣的分析物转移到 NMR 光谱仪之前，使用在线固相萃取（SPE）装置对 LC 后捕获的分析物（LC-SPE-NMR）进行分析，这是一种强大的装置，可以显著减少生成高质量异核 NMR 位移相关数据所需的分析物数量。本文将讨论不同的技术实现方案，并提供来自天然产物研究和工业应用的典型实例。
