INRA, MICALIS, UMR1319, 78352 Jouy-en-Josas cédex, France.
Curr Opin Microbiol. 2012 Jun;15(3):381-9. doi: 10.1016/j.mib.2012.04.003. Epub 2012 May 30.
The study of health-beneficial effects that probiotic bacteria can exert on humans and animals is at its beginning. Pending scientific questions include the identification of molecular markers of the health-promoting activity of specific strains, which may be used to select novel probiotic strains and to gain understanding of the mechanisms underlying their effects. In that perspective, the role of bacterial proteins must be evaluated, placing proteomics-based approaches at the core of the field. Until now, most proteomic analyses focused on the dynamics of abundant cytoplasmic proteins during adaptation of bacteria to conditions mimicking the gastro-intestinal tract environment. The development of in silico and experimental procedures allowing identification and quantification of surface-exposed and secreted proteins should boost our understanding of bacteria-host crosstalk.
对益生菌对人类和动物有益健康的影响的研究才刚刚开始。悬而未决的科学问题包括鉴定特定菌株促进健康活动的分子标记物，这可用于选择新型益生菌菌株并深入了解其作用机制。从这个角度来看，必须评估细菌蛋白的作用，将基于蛋白质组学的方法置于该领域的核心位置。到目前为止，大多数蛋白质组学分析都集中在细菌适应模拟胃肠道环境条件时细胞质中丰富蛋白的动态变化上。开发允许识别和定量表面暴露和分泌蛋白的计算和实验程序，应有助于我们理解细菌与宿主的相互作用。
