含神经氨酸酶的 M1 流感 VLPs 诱导异源型交叉保护。
Influenza M1 VLPs containing neuraminidase induce heterosubtypic cross-protection.
机构信息
Department of Medical Zoology, Kyung Hee University School of Medicine, Seoul 130-701, Korea.
出版信息
Virology. 2012 Sep 1;430(2):127-35. doi: 10.1016/j.virol.2012.05.006. Epub 2012 Jun 2.
Abstract
Influenza virus like particles (VLPs) containing hemagglutinin were previously demonstrated to induce protection against the homologous strains. However, little information is available on the protective role of neuraminidase (NA), the second major glycoprotein. In this study, we developed VLPs (NA VLPs) containing NA and M1 derived from A/PR/8/34 (H1N1) influenza virus, and investigated their ability to induce protective immunity. Intranasal immunization with NA VLPs induced serum antibody responses to H1N1 and H3N2 influenza A viruses as well as significant neuraminidase inhibition activity. Importantly, mice immunized with NA VLPs were 100% protected against lethal infection by the homologous A/PR/8/34 (H1N1) as well as heterosubtypic A/Philippines/82 (H3N2) virus, although body weight loss was observed after lethal challenge with heterosubtypic H3N2 virus. The present study therefore provides evidence that influenza VLPs containing M1 and NA are capable of inducing immunity to homologous as well as antigenically distinct influenza A virus strains.
摘要
含有血凝素的流感病毒样颗粒（VLPs）先前已被证明能诱导针对同源株的保护作用。然而，关于神经氨酸酶（NA）的保护作用的信息很少，NA 是第二种主要糖蛋白。在本研究中，我们开发了含有源自 A/PR/8/34（H1N1）流感病毒的 NA 和 M1 的 VLPs（NA VLPs），并研究了它们诱导保护性免疫的能力。鼻内免疫 NA VLPs 可诱导针对甲型流感病毒 H1N1 和 H3N2 的血清抗体反应以及显著的神经氨酸酶抑制活性。重要的是，用 NA VLPs 免疫的小鼠对同源 A/PR/8/34（H1N1）以及异源 H3N2 病毒（A/Philippines/82）的致死性感染均 100%得到保护，尽管在致死性异源 H3N2 病毒攻击后观察到体重减轻。因此，本研究提供了证据表明，含有 M1 和 NA 的流感 VLPs 能够诱导对同源和抗原上不同的甲型流感病毒株的免疫。
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