Department of Hematology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
Immunobiology. 2012 Aug;217(8):816-22. doi: 10.1016/j.imbio.2012.05.004. Epub 2012 May 11.
The immune modulatory effect of granulocyte colony-stimulating factor (G-CSF) on T cells resulted in an unexpected low incidence of graft-versus-host disease (GVHD) in allogeneic peripheral blood stem cell transplantation (allo-PBSCT). Recently, αβ(+) T cells are identified as the primary effector cells for GVHD. However, whether G-CSF could influence the repertoire of αβ(+) T cells (TRAV and TRBV repertoire) and CD3 genes remains unclear. To further characterize this feature, we investigated the effect of G-CSF mobilization on the T cell receptors (TCR) of αβ(+) T cells (TRAV and TRBV repertoire) and CD3 genes, as well as the association between the changes of TCR repertoire and GVHD in patients undergoing G-CSF mobilized allo-PBSCT. We found that G-CSF mobilization had an effect on the expression patterns, clonality and signal transduction of TRAV and TRBV repertoire. This alteration might play a role in mediating GVHD in G-CSF mobilized allo-PBSCT.
粒细胞集落刺激因子 (G-CSF) 对 T 细胞的免疫调节作用导致异基因外周血造血干细胞移植 (allo-PBSCT) 中移植物抗宿主病 (GVHD) 的发生率出人意料地低。最近，αβ(+) T 细胞被鉴定为 GVHD 的主要效应细胞。然而，G-CSF 是否会影响 αβ(+) T 细胞（TRAV 和 TRBV 库）和 CD3 基因的库尚不清楚。为了进一步阐明这一特征，我们研究了 G-CSF 动员对接受 G-CSF 动员的 allo-PBSCT 患者 T 细胞受体 (TCR) 的 αβ(+) T 细胞（TRAV 和 TRBV 库）和 CD3 基因的影响，以及 TCR 库变化与 GVHD 之间的关系。我们发现 G-CSF 动员对 TRAV 和 TRBV 库的表达模式、克隆性和信号转导有影响。这种改变可能在 G-CSF 动员的 allo-PBSCT 中介导 GVHD 中发挥作用。
