1 Institute of Hematology, Jinan University , Guangzhou, China .
DNA Cell Biol. 2014 Mar;33(3):182-8. doi: 10.1089/dna.2013.2277. Epub 2014 Jan 11.
Graft versus host disease (GVHD) is the main complication following allogeneic hematopoietic stem cell transplantation (allo-HSCT). Recent data indicated that regulatory T (Treg) cells might relate to GVHD, and such functions might be mediated by certain T-cell receptor (TCR) subfamily of Treg cells. Thus, we analyzed the distribution and clonality of the TCR Vα and Vβ repertoire of Treg cells from leukemia patients with and without GVHD after allo-HSCT. Numerous TCR Vα subfamilies, including Vα1, Vα9, Vα13, Vα16-19, and Vα24-29, were absent in Treg cells after allo-HSCT. The usage numbers for the TCR Vα and Vβ subfamilies in Treg cells from patients without GVHD appeared more widely. The expression frequencies of Vα10 or Vα20 between both groups were significantly different. Moreover, the expression frequency of TCR Vβ2 subfamily in patients without GVHD was significantly higher than that in patients with GVHD. Oligoclonally expanded TCR Vα and Vβ Treg cells were identified in a few samples in both groups. Restricted utilization of the Vα and Vβ subfamilies and the absence of some important TCR rearrangements in Treg cells may be related to GVHD due to a lower regulating function of Treg subfamilies.
移植物抗宿主病（GVHD）是异基因造血干细胞移植（allo-HSCT）后主要并发症。最近的数据表明，调节性 T（Treg）细胞可能与 GVHD 有关，并且这种功能可能由 Treg 细胞的某些 T 细胞受体（TCR）亚家族介导。因此，我们分析了白血病患者 allo-HSCT 后有无 GVHD 时 Treg 细胞 TCR Vα 和 Vβ 受体库的分布和克隆性。在 allo-HSCT 后，Treg 细胞中存在许多 TCR Vα 亚家族，包括 Vα1、Vα9、Vα13、Vα16-19 和 Vα24-29。无 GVHD 患者的 Treg 细胞 TCR Vα 和 Vβ 亚家族的使用数量出现更广泛。两组间 Vα10 或 Vα20 的表达频率差异显著。此外，无 GVHD 患者的 TCR Vβ2 亚家族表达频率明显高于有 GVHD 患者。两组中少数样本中鉴定出 TCR Vα 和 Vβ 寡克隆扩增 Treg 细胞。Treg 细胞 TCR Vα 和 Vβ 亚家族的有限利用和某些重要 TCR 重排的缺失可能与 GVHD 有关，因为 Treg 亚家族的调节功能降低。
