Virus Laboratory, The Affiliated Shengjing Hospital, China Medical University, 110004 Shenyang, Liaoning, China.
Infect Genet Evol. 2012 Oct;12(7):1466-72. doi: 10.1016/j.meegid.2012.05.004. Epub 2012 May 29.
Infection with certain types of human papillomaviruses (HPVs) is a risk factor for the development of cervical cancer. HPV type 58 (HPV 58) is prevalent among Chinese women. The intratype sequence variants differ in oncogenic potential and their prevalences vary across geographic regions. The objective of this study was to analyze the variations of HPV 58 E6, E7, L1 genes and long control region (LCR) in a large samples collected from northeastern Chinese women with cervical lesions. A total of 2938 cervical samples were collected and tested for HPV type using a chip hybridization assay. The E6, E7, L1 genes and LCR of HPV 58 strains were amplified and the amplicons were subjected to direct nucleotide sequencing for variation identification. A total of 235 specimens were HPV 58 positive. High proportions of HPV 58 E6 (83.8%), E7 (76.7%), L1 (90.8%) genes and LCR (91.4%) variants were identified in strains from Chinese women. The most frequently observed variations were C307T (52.4%) in E6, T744G (74.9%) in E7, A6014C (56.9%) in L1 genes and C7266T, A7714G (55.2%) in LCR. For the E6 gene, nine nucleotide variations were identified. Among them, the A140G (T11A), A184C (E25D), G266C (V53L) and A313G were novel variations. Sequencing of the E7 gene revealed four typical nucleotide changes: G761A (G63D), G694A (G41R), T803C (V77A) and T744G. In the L1 gene, 39 nucleotide variations and 13 amino acid substitutions were identified. Among these mutations, 21 variations are reported here for the first time. Lineage A of HPV 58 was found in 142 of 174 strains (81.6%). The most prevalent HPV 58 variants in Chinese northeastern women belongs to lineage A. Novel variations in E6 and L1 genes were also reported. These findings provide new data regarding E6 and L1 gene variations of HPV 58 from women in northeast China.
某些类型的人乳头瘤病毒（HPV）感染是宫颈癌发展的一个风险因素。HPV 58 型（HPV 58）在中国女性中较为普遍。同型内序列变异在致癌潜力上存在差异，其流行率在不同地理区域也有所不同。本研究的目的是分析从中国东北地区宫颈病变女性中采集的大量样本中 HPV 58 E6、E7、L1 基因和长控制区（LCR）的变异情况。共采集了 2938 例宫颈样本，使用芯片杂交试验检测 HPV 类型。扩增 HPV 58 株的 E6、E7、L1 基因和 LCR，并对扩增子进行直接核苷酸测序以鉴定变异。共有 235 例标本 HPV 58 阳性。在中国女性的 HPV 58 株中发现 E6（83.8%）、E7（76.7%）、L1（90.8%）基因和 LCR（91.4%）变异的比例较高。最常见的变异是 E6 中的 C307T（52.4%）、E7 中的 T744G（74.9%）、L1 基因中的 A6014C（56.9%）和 LCR 中的 C7266T、A7714G（55.2%）。E6 基因共发现 9 个核苷酸变异，其中 A140G（T11A）、A184C（E25D）、G266C（V53L）和 A313G 为新变异。E7 基因测序显示有 4 个典型的核苷酸变化：G761A（G63D）、G694A（G41R）、T803C（V77A）和 T744G。L1 基因共发现 39 个核苷酸变异和 13 个氨基酸取代，其中 21 个变异为首次报道。174 株中的 142 株（81.6%）属于 HPV 58 谱系 A。在中国东北女性中最常见的 HPV 58 变异株属于谱系 A。还报道了 E6 和 L1 基因的新变异。这些发现为中国东北地区女性 HPV 58 的 E6 和 L1 基因变异提供了新的数据。
