College of Food and Drugs, Luoyang Polytechnic, Luoyang, Henan, China.
College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, China.
Sci Rep. 2023 Feb 8;13(1):2252. doi: 10.1038/s41598-022-24641-4.
The present study aims to investigate the genotype distribution of Human papillomavirus (HPV) and variations of HPV18 and HPV58 infection among 6538 females in Luoyang city during 2019-2021. The overall positive rate of females with HPV infection was 12.34%, with 9.74% were infected with single HPV and 2.60% with multiple HPVs. The prevalent rate of high-risk HPV (HR-HPV) was 9.85% and the top five HR-HPV genotypes were HPV52 (1.94%), HPV16 (1.93%), HPV58 (1.48%), HPV51 (1.02%) and HPVV39 (0.99%). Two peaks of HPV infections rates were observed in females aged ≤ 20 and 61-65 years old. To characterize mutations, 39 HPV18 and 56 HPV58 L1, E6 and E7 genes were sequenced and submitted to GenBank. In the HPV18 E6-E7-L1 sequences, 38 nucleotides changes were observed with 10/38 were non-synonymous mutations (5 in E6 gene, 1 in E7 gene and 4 in L1 gene). In the HPV58 E6-E7-L1 sequences, 53 nucleotides changes were observed with 23/53 were non-synonymous mutations (3 in E6 gene, 5 in E7 gene and 15 in L1 gene). Phylogenetic analysis based on L1 gene showed that 92.3% (36/39) of HPV18 isolates fell into sublineage A1 and 7.7% (3/39) belonged to A5. For HPV58, 75.0% (42/56) isolates belonged to sublineage A1 and 25.0% (14/56) were sublineage A2. There was no association between amino mutation and cervical lesions. The present study provides basic information about the distribution, genotypes and variations of HPV among females population in Luoyang city, which would assist in the formulation of HPV screening and vaccination programs and preventive strategies for HPV-attributable cancer in this region.
本研究旨在调查 2019-2021 年洛阳市 6538 名女性中人类乳头瘤病毒（HPV）的基因型分布和 HPV18 和 HPV58 感染的变异情况。女性 HPV 感染的总阳性率为 12.34%，其中 9.74%感染单一 HPV，2.60%感染多种 HPV。高危型 HPV（HR-HPV）的流行率为 9.85%，前五种 HR-HPV 基因型为 HPV52（1.94%）、HPV16（1.93%）、HPV58（1.48%）、HPV51（1.02%）和 HPV V39（0.99%）。女性 HPV 感染率有两个高峰，年龄分别为≤20 岁和 61-65 岁。为了描述突变特征，对 39 株 HPV18 和 56 株 HPV58 的 L1、E6 和 E7 基因进行了测序，并提交给 GenBank。在 HPV18 E6-E7-L1 序列中，观察到 38 个核苷酸变化，其中 10/38 为非同义突变（E6 基因 5 个，E7 基因 1 个，L1 基因 4 个）。在 HPV58 E6-E7-L1 序列中，观察到 53 个核苷酸变化，其中 23/53 为非同义突变（E6 基因 3 个，E7 基因 5 个，L1 基因 15 个）。基于 L1 基因的系统进化分析显示，92.3%（36/39）的 HPV18 分离株属于亚谱系 A1，7.7%（3/39）属于 A5。对于 HPV58，75.0%（42/56）的分离株属于亚谱系 A1，25.0%（14/56）属于 A2。氨基酸突变与宫颈病变无相关性。本研究为洛阳市女性人群 HPV 的分布、基因型和变异提供了基础信息，有助于制定该地区 HPV 筛查和疫苗接种计划以及 HPV 相关癌症的预防策略。
