Department of Laboratory Medicine, Jingzhou Hospital, Yangtze University, Jingzhou, 434020, China.
Department of Immunology, School of Medicine, Yangtze University, Jingzhou, China.
Virol J. 2022 Apr 22;19(1):71. doi: 10.1186/s12985-022-01801-6.
Cervical cancer is a common malignant tumor in women, with a high mortality rate, has great harm to women's health. Long-term and persistent infection of high-risk human papillomavirus (HR-HPV) is the main reason of the occurrence and development of cervical cancer.
The infection rate of HPV-58 is higher in the Jingzhou area. In this study, 172 complete HPV-58 E6-E7 sequences were amplified by polymerase chain reaction (PCR), the amplified products were sequenced, and the gene variations of HPV-58 E6-E7 were analyzed. A Neighbor-Joining phylogenetic tree was constructed by MEGA 11. The secondary structure of E6 and E7 protein was investigated. PAML X was used to analyze the selective pressure. The B cell epitopes of E6 and E7 proteins in HPV-58 were predicted by ABCpred server.
In E6 sequences, 10 single nucleotide variants were observed, including 7 synonymous and 3 non-synonymous variants. In E7 sequences, 12 single nucleotide variants were found, including 3 synonymous variants and 9 non-synonymous variants. There are 5 novel variants. The phylogenetic analysis showed that all the E6-E7 sequences were distributed in A lineage. No positively selected site was found in E6 sequence, but G63 in E7 sequences was identified as positively selected site. Some amino acid substitutions affected multiple B cell epitopes.
Various E6 and E7 mutational data may prove useful for development of better diagnostic and vaccines for the region of Jingzhou, Hubei province of central China.
宫颈癌是女性常见的恶性肿瘤，死亡率较高，对女性健康危害极大。高危型人乳头瘤病毒（HR-HPV）的长期持续感染是宫颈癌发生发展的主要原因。
本研究采用聚合酶链反应（PCR）扩增了 172 株完整的 HPV-58 E6-E7 序列，对扩增产物进行测序，并分析 HPV-58 E6-E7 的基因变异情况。应用 MEGA 11 构建 Neighbor-Joining 系统进化树，分析 E6 和 E7 蛋白的二级结构，采用 PAML X 分析 E6 和 E7 蛋白的选择压力，利用 ABCpred 服务器预测 HPV-58 E6 和 E7 蛋白的 B 细胞表位。
E6 序列中观察到 10 个单核苷酸变异，包括 7 个同义变异和 3 个非同义变异；E7 序列中发现 12 个单核苷酸变异，包括 3 个同义变异和 9 个非同义变异，发现 5 个新的变异。系统进化分析显示，所有 E6-E7 序列均分布在 A 谱系中。E6 序列中未发现正选择位点，但 E7 序列中的 G63 被鉴定为正选择位点。一些氨基酸取代影响多个 B 细胞表位。
各种 E6 和 E7 突变数据可能有助于为中国中部湖北省荆州市地区开发更好的诊断和疫苗。
