Leite Rodrigo P, Predes Fabrícia S, Monteiro Juliana C, Freitas Karine M, Wada Ronaldo S, Dolder Heidi
Department of Cell Biology, Institute of Biology, UNICAMP, São Paulo, Brazil.
Toxicol Pathol. 2013 Jan;41(1):73-9. doi: 10.1177/0192623312447541. Epub 2012 Jun 1.
Paullinia cupana is an Amazonian bush whose seeds have long been used in folk medicine. However, most of the therapeutic properties attributed to this plant are broad and nonspecific, although an antioxidant activity has been reported. On the other hand, cadmium is a heavy metal known for increasing free radicals, hence resulting in cellular oxidative damages. This study was designed to evaluate whether Paullinia cupana is able to reduce cadmium-induced morphological impairment in Wistar rat testis. Adult male Wistar rats 110 days old were ip injected with cadmium (1.15 mg/kg BW [body weight]) and subsequently treated with P. cupana during 56 days. Furthermore, groups receiving either P. cupana extract or cadmium are mentioned. After the treatment period, testis samples were subjected to histological and stereological analyses. Moderate to severe testicular impairments were shown by the animals exposed to cadmium. However, the animals supplemented with P. cupana after cadmium exposure showed a significant decrease in the proportion of damaged seminiferous tubules. Also, P. cupana supplementation was effective in maintaining the number of Leydig cells per testis in the animals exposed to cadmium. In conclusion, P. cupana supplementation was partially efficient in preventing cadmium from damaging the testis of adult Wistar rats.
瓜拉那是一种亚马逊灌木，其种子长期以来一直用于民间医学。然而，尽管已报道该植物具有抗氧化活性，但归因于这种植物的大多数治疗特性都很宽泛且不具有特异性。另一方面，镉是一种以增加自由基而闻名的重金属，从而导致细胞氧化损伤。本研究旨在评估瓜拉那是否能够减轻镉诱导的Wistar大鼠睾丸形态损伤。110日龄的成年雄性Wistar大鼠腹腔注射镉（1.15毫克/千克体重），随后在56天内用瓜拉那进行治疗。此外，还提到了接受瓜拉那提取物或镉的组。治疗期结束后，对睾丸样本进行组织学和体视学分析。暴露于镉的动物表现出中度至重度睾丸损伤。然而，镉暴露后补充瓜拉那的动物受损生精小管的比例显著降低。此外，补充瓜拉那有效地维持了暴露于镉的动物每个睾丸中Leydig细胞的数量。总之，补充瓜拉那在预防镉对成年Wistar大鼠睾丸的损伤方面部分有效。
