Arantes Leticia Priscilla, Peres Tanara Vieira, Chen Pam, Caito Samuel, Aschner Michael, Soares Félix Alexandre Antunes
Departamento de Bioquímica e Biologia Molecular, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.
Department of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, NY, United States.
Toxicol Res (Camb). 2016 Nov 1;5(6):1629-1638. doi: 10.1039/C6TX00161K. Epub 2016 Aug 24.
The influence of routine guarana () consumption on apparent tolerance to mercury intoxication has been proposed. The present study investigated this hypothesis in , a suitable experimental model for studies in toxicology. Wild type (WT) and worm strains were pretreated with guarana ethanolic extract (GEE) from larvae 1 (L1) to L4 stage and then exposed for 6 hours to methylmercury (MeHg). The analyses included evaluation of GEE's effects on lethality, developmental delay, feeding, locomotion, gene expression (, , , , , , and ) and antioxidant activity. GEE pre-treatment had no aberrant effects on WT worms exposed to MeHg, and protected worms which are more susceptible to environmental stresses. Protective effects of GEE might be dependent on modulation of genes other than those directly involved in antioxidant activity. GEE increased the expression of genes involved in metal transport (), metal detoxification ( and ) and antioxidant responses ( and ). Thus, routine consumption of guarana might be beneficial in protecting against MeHg-induced toxicity.
有人提出日常食用瓜拉那（）对汞中毒表观耐受性的影响。本研究在一种适合毒理学研究的实验模型中对这一假设进行了研究。野生型（WT）和 蠕虫品系从幼虫1（L1）到L4阶段用瓜拉那乙醇提取物（GEE）进行预处理，然后暴露于甲基汞（MeHg）6小时。分析包括评估GEE对致死率、发育延迟、摄食、运动、基因表达（、、、、、、 和 ）以及抗氧化活性的影响。GEE预处理对暴露于MeHg的WT蠕虫没有异常影响，并保护了对环境压力更敏感的 蠕虫。GEE的保护作用可能取决于对除直接参与抗氧化活性的基因之外的其他基因的调节。GEE增加了参与金属转运（）、金属解毒（ 和 ）以及抗氧化反应（ 和 ）的基因的表达。因此，日常食用瓜拉那可能有助于预防MeHg诱导的毒性。
