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[三明水稻显性核雄性不育基因的定位]

[Mapping of Sanming dominant genic male sterility gene in rice].

作者信息

Yang Ze-Mao, Xie Xiao-Fang, Huang Xian-Bo, Wang Feng-Qing, Tong Zhi-Jun, Duan Yuan-Ling, Lan Tao, Wu Wei-Ren

机构信息

College of Agriculture & Biotechnology, Zhejiang University, Hangzhou 310000, China.

出版信息

Yi Chuan. 2012 May;34(5):615-20. doi: 10.3724/sp.j.1005.2012.00615.

Abstract

The mutant of "Sanming Dominant Genic Male Sterile Rice" was found from an F2 population of cross "SE2lS/Basmati370" by Sanming Institute of Agricultural Science in 2001. It has proven that the male sterility of this mutant is controlled by a dominant gene (named as SMS). By multiple backcrosses, this dominant male sterile allele was introduced into the genetic background of an indica rice cultivar Jiafuzhan (which was known as Jiabuyu). In order to map SMS, a mapping population was constructed by crossing Jiabuyu with a japonica cultivar Nipponbare and further crossing the F1 with Jiafuzhan. By bulked segregant analysis and linkage analysis using SSR and INDEL markers, SMS was mapped to a 99 kb interval between INDEL markers ZM30 and ZM9 on chromosome 8. This result will facilitate cloning of SMS.

摘要

2001年,三明市农业科学研究所在“SE2lS/巴斯马蒂370”杂交组合的F2群体中发现了“三明显性核雄性不育水稻”的突变体。已证明该突变体的雄性不育受一个显性基因(命名为SMS)控制。通过多次回交,将这个显性雄性不育等位基因导入到籼稻品种佳辐占(曾用名加卜玉)的遗传背景中。为了定位SMS,通过将佳辐占与粳稻品种日本晴杂交,然后将F1与佳辐占进一步杂交构建了一个定位群体。利用SSR和INDEL标记通过混合分组分析法和连锁分析,将SMS定位到第8号染色体上INDEL标记ZM30和ZM9之间99 kb的区间内。这一结果将有助于SMS的克隆。

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