Jiangsu Key Laboratory of Crop Genetics and Physiology/Key Laboratory of the Ministry of Education for Plant Functional Genomics, Yangzhou University, 88 Daxue Road, 225009, Yangzhou, Jiangsu Province, People's Republic of China.
Theor Appl Genet. 2011 Feb;122(2):365-72. doi: 10.1007/s00122-010-1452-0. Epub 2010 Oct 12.
Photoperiod-thermo-sensitive genic male sterile (PTGMS) rice exhibits a number of desirable traits for hybrid rice production. The cloning genes responsible for PTGMS and those elucidating male sterility mechanisms and reversibility to fertility would be of great significance to provide a foundation to develop new male sterile lines. Guangzhan63S, a PTGMS line, is one of the most widely used indica two-line hybrid rice breeding systems in China. In this study, genetic analysis based on F(2) and BC(1)F(2) populations derived from a cross between Guangzhan63S and 1587, determined a single recessive gene controls male sterility in Guangzhan63S. Molecular marker techniques combined with bulked-segregant analysis (BSA) were used and located the target gene (named ptgms2-1) between two SSR markers RM12521 and RM12823. Fine mapping of the ptgms2-1 locus was conducted with 45 new Insertion-Deletion (InDel) markers developed between the RM12521 and RM12823 region, using 634 sterile individuals from F(2) and BC(1)F(2) populations. Ptgms2-1 was further mapped to a 50.4 kb DNA fragment between two InDel markers, S2-40 and S2-44, with genetic distances of 0.08 and 0.16 cM, respectively, which cosegregated with S2-43 located on the AP004039 BAC clone. Ten genes were identified in this region based on annotation results from the RiceGAAS system. A nuclear ribonuclease Z gene was identified as the candidate for the ptgms2-1 gene. This result will facilitate cloning the ptgms2-1 gene. The tightly linked markers for the ptgms2-1 gene locus will further provide a useful tool for marker-assisted selection of this gene in rice breeding programs.
光温敏核雄性不育（PTGMS）水稻表现出许多适合杂交水稻生产的优良特性。克隆与 PTGMS 相关的基因，以及阐明雄性不育机制及其育性恢复的可逆性，对于开发新的雄性不育系具有重要意义。广占 63S 是中国应用最广泛的籼型两系杂交水稻育种系统之一。本研究基于广占 63S 与 1587 杂交产生的 F2 和 BC1F2 群体的遗传分析，确定了一个单隐性基因控制广占 63S 的雄性不育。利用分子标记技术结合分离群体分组分析法（BSA），将目标基因（命名为 ptgms2-1）定位在两个 SSR 标记 RM12521 和 RM12823 之间。利用在 RM12521 和 RM12823 区域之间开发的 45 个新的插入/缺失（InDel）标记，对 ptgms2-1 基因座进行了精细定位，使用来自 F2 和 BC1F2 群体的 634 个不育个体。Ptgms2-1 进一步定位在两个 InDel 标记 S2-40 和 S2-44 之间的 50.4kb DNA 片段上，遗传距离分别为 0.08 和 0.16cM，与位于 AP004039 BAC 克隆上的 S2-43 共分离。根据 RiceGAAS 系统注释结果，在该区域鉴定出 10 个基因。一个核核糖核酸酶 Z 基因被鉴定为 ptgms2-1 基因的候选基因。这一结果将有助于克隆 ptgms2-1 基因。与 ptgms2-1 基因座紧密连锁的标记将进一步为水稻育种计划中该基因的标记辅助选择提供有用的工具。
