Department of Chemistry and The Photonics Center, Boston University, Boston, MA 02215, USA.
Nanoscale. 2012 Jul 7;4(13):3917-25. doi: 10.1039/c2nr30747b. Epub 2012 Jun 1.
We introduce a surface enhanced Raman spectroscopy (SERS) stamping approach for acquiring cell-surface specific vibrational spectra of individual living cells under physiological conditions. The SERS stamping approach utilizes a nanostructured metal surface on top of a lithographically defined piston that can be translated in 3-dimensions with nanometer resolution to contact living cells in solution with a pristine metal surface. We applied this approach to characterize the chemical composition of the cellular surface of living MCF7 breast cancer cells and to monitor its change upon addition of the enzyme hyaluronidase, which degrades major constituents of the pericellular matrix. Although the cell surface spectra show significant cell-to-cell fluctuations, a statistical barcode analysis of the spectra ensembles reveals systematic changes in the cell surface SERS spectra upon addition of hyaluronidase, which are consistent with a thinning of the pericellular matrix.
我们介绍了一种表面增强拉曼光谱（SERS）压印方法，用于在生理条件下获取单个活细胞表面的特定振动光谱。SERS 压印方法利用了顶部具有纳米结构的金属表面，该表面位于光刻定义的活塞上，可以在 3 维空间中以纳米分辨率平移，以与溶液中的活细胞用原始金属表面接触。我们应用此方法来表征活 MCF7 乳腺癌细胞的细胞表面化学组成，并监测在添加酶透明质酸酶后其变化，透明质酸酶会降解细胞外基质的主要成分。尽管细胞表面光谱显示出显著的细胞间波动，但对光谱集合的统计条形码分析表明，在添加透明质酸酶后，细胞表面 SERS 光谱会发生系统变化，这与细胞外基质的变薄一致。
