Sairam M R
J Endocrinol. 1979 Aug;82(2):253-62. doi: 10.1677/joe.0.0820253.
A modified bovine testicular receptor was used to evaluate highly purified follicle-stimulating hormone (FSH) from a number of species. The particulate receptor obtained from adult bovine testes could be stored frozen or lyophilized for long periods without appreciable decrease in the binding of the ligand facility or in loss of specificity. The bovine testis receptor binds twice as much 125I-labelled ovine FSH as 125I-labelled human hormone. When FSH from different species was compared against NIH-FSH-S10, using various FSH ligands, the ovine hormone was clearly the most active, although many had comparable in-vivo biological potencies. The results suggest that there is probably some species specificity in the hormone-receptor interactions. As the ovine hormone is structurally closer to the bovine, from which the receptor was derived, it appears to have the highest activity in vitro. Marked differences in the biological activities of the different preparations between the human chorionic gonadotrophin-augmentation test and the in-vitro assays have been observed. In the in-vitro assays, all preparations, with the exception of the porcine hormone preparation, were less active and the ratio of bioassay/radioreceptor assay varied widely. In the radioreceptor assays, all FSH preparations except pregnant mare serum gonadotrophin (PMSG) showed parallel inhibition curves. The three different PMSG preparations examined gave inhibition lines that were parallel to each other.
一种经过改良的牛睾丸受体被用于评估来自多个物种的高度纯化的促卵泡激素（FSH）。从成年牛睾丸获得的颗粒状受体可以长期冷冻保存或冻干，而其与配体结合的能力及特异性不会有明显下降。牛睾丸受体与125I标记的绵羊FSH的结合量是与125I标记的人促卵泡激素结合量的两倍。当使用各种FSH配体将不同物种的FSH与NIH-FSH-S10进行比较时，绵羊促卵泡激素显然活性最高，尽管许多FSH在体内具有相当的生物学效价。结果表明，激素与受体的相互作用可能存在某种物种特异性。由于绵羊促卵泡激素在结构上与受体来源的牛促卵泡激素更接近，它在体外似乎具有最高活性。已观察到不同制剂在人绒毛膜促性腺激素增强试验和体外试验中的生物学活性存在显著差异。在体外试验中，除猪促卵泡激素制剂外，所有制剂的活性都较低，生物测定/放射受体测定的比值差异很大。在放射受体测定中，除孕马血清促性腺激素（PMSG）外，所有FSH制剂均呈现平行抑制曲线。所检测的三种不同PMSG制剂给出的抑制曲线相互平行。
