大鼠促卵泡激素放射受体测定和放射免疫测定的物种特异性。

Species specificity of radioreceptor assay and radioimmunoassay for rat FSH.

作者信息

Minegishi T, Mizunuma H, Igarashi M, Wakabayashi K

出版信息

Endocrinol Jpn. 1981 Jun;28(3):369-73. doi: 10.1507/endocrj1954.28.369.

DOI:10.1507/endocrj1954.28.369

Abstract

Species specificity of the radioreceptor assay (RRA) for rat FSH, in which pregnant mare serum gonadotropin (PMSG)-treated immature rat ovary was employed as the receptor, was compared with that of NIAMDD rat FSH radioimmunoassay (RIA). In the RIA system, pituitary preparations from mammals only showed significant crossreaction. Their inhibition curves, however, were not always parallel to the standard curve. On the other hand, in the RRA system, the pituitary preparations from mammals, avians, lizard and amphibians competitively inhibited the binding of radioactive rat FSH to the ovarian receptor. Only the pituitary preparation from dog salmon failed to show any crossreaction in the RRA system. These results indicated that this RRA system would be useful for the measurement of FSH or gonadotropins of the pituitaries from mammals to amphibians.

摘要

以经孕马血清促性腺激素（PMSG）处理的未成熟大鼠卵巢作为受体，对大鼠促卵泡激素（FSH）放射受体分析（RRA）的物种特异性与国立卫生研究院糖尿病、消化和肾脏疾病研究所（NIAMDD）大鼠FSH放射免疫分析（RIA）的物种特异性进行了比较。在RIA系统中，仅来自哺乳动物的垂体提取物显示出显著的交叉反应。然而，它们的抑制曲线并不总是与标准曲线平行。另一方面，在RRA系统中，来自哺乳动物、鸟类、蜥蜴和两栖动物的垂体提取物竞争性抑制放射性大鼠FSH与卵巢受体的结合。在RRA系统中，仅狗鲑的垂体提取物未显示出任何交叉反应。这些结果表明，该RRA系统可用于测定从哺乳动物到两栖动物垂体中的FSH或促性腺激素。