Findley W E, Steinberger A
Endocrinology. 1983 Sep;113(3):1081-7. doi: 10.1210/endo-113-3-1081.
A homologous radioreceptor assay (RRA) for rat FSH (rFSH), which is both sensitive and easy to perform, is described. The receptor preparation is isolated from a 12,000 X G pellet of rat testes homogenate prepared with a high speed tissue grinder using Tris buffer. The sensitivity of the assay extends below 10 ng rFSH RP-1 (approximately 0.13 ng purified I-3 rat FSH), and the range of the assay spans 2 orders of magnitude. The specific binding of the [125I]rFSH tracer is 22-28% of the total tracer added. Such binding, which exceeds previously published values by 2- to 4-fold, allows the addition of relatively small amounts of total tracer radioactivity, which, in turn, contributes to a low nonspecific binding and highly reproducible values for replicates (coefficient of variation, approximately 3.0%). This represents the first homologous RRA for rFSH using testicular receptors. Likewise, the sensitivity and reproducibility exceed those of previous RRAs for rFSH.
本文描述了一种用于大鼠促卵泡激素（rFSH）的同源放射受体分析（RRA）方法，该方法灵敏且易于操作。受体制剂是从用高速组织研磨机在Tris缓冲液中制备的大鼠睾丸匀浆的12,000×g沉淀中分离得到的。该分析方法的灵敏度可低至10 ng rFSH RP-1以下（约0.13 ng纯化的I-3大鼠FSH），分析范围跨越2个数量级。[125I]rFSH示踪剂的特异性结合占添加的总示踪剂的22%-28%。这种结合比先前发表的值高出2至4倍，使得能够添加相对少量的总示踪剂放射性，这反过来又有助于降低非特异性结合，并使重复测量值具有高度可重复性（变异系数约为3.0%）。这是首次使用睾丸受体进行的rFSH同源RRA。同样，该方法的灵敏度和可重复性超过了先前用于rFSH的RRA。
