Pérez-Vich B, Garcés R, Fernández-Martínez J M
Instituto de Agricultura Sostenible, CSIC, Apartado 4084, E-14080, Córdoba, Spain, ES.
Theor Appl Genet. 1999 Aug;99(3-4):663-9. doi: 10.1007/s001220051282.
A sunflower mutant, CAS-3, with about 25% stearic acid (C18:0) in the seed oil was recently isolated after a chemical-mutagen treatment of RDF-1-532 seeds (8% C18:0). To study the inheritance of the high C18:0 content, CAS-3 was reciprocally crossed to RDF-1-532 and HA-89 (5% C18:0). Significant reciprocal-cross differences were found in one of the two crosses, indicating possible maternal effects. In the CAS-3 and RDF-1-532 crosses, the segregation patterns of the F(1), BC(1), and F(2) populations fitted a one-locus (designated Es1) model with two alleles (Es1, es1) and with partial dominance of low over high C18:0 content. Segregation patterns in the CAS-3 and HA-89 crosses indicated the presence of a second independent locus (designated Es2) with two alleles (Es2, es2), also with partial dominance of low over high C18:0 content. From these results, the proposed genotypes (C18:0 content) of each parent were as follows: CAS-3 (25.0% C18:0) =es1es1es2es2; RDF-1-532 (8.0% C18:0) =Es1Es1es2es2; and HA-89 (4.6% C18:0) =Es1Es1Es2Es2. The relationship between the proposed genotypes and their C18:0 content indicates that the Es1 locus has a greater effect on the C18:0 content than the Es2 locus. Apparently, the mutagenic treatment caused a mutation of Es1 to es1 in RDF-1-532.
最近，在对RDF - 1 - 532种子（硬脂酸含量为8%，即C18:0）进行化学诱变处理后，分离出了一种向日葵突变体CAS - 3，其种子油中硬脂酸（C18:0）含量约为25%。为研究高C18:0含量的遗传特性，将CAS - 3与RDF - 1 - 532和HA - 89（C18:0含量为5%）进行正反交。在这两个杂交组合中的其中一个发现了显著的正反交差异，表明可能存在母性效应。在CAS - 3与RDF - 1 - 532的杂交中，F(1)、BC(1)和F(2)群体的分离模式符合一个单基因座（命名为Es1）模型，该模型有两个等位基因（Es1和es1），且低C18:0含量对高C18:0含量呈部分显性。CAS - 3与HA - 89杂交的分离模式表明存在第二个独立的基因座（命名为Es2），有两个等位基因（Es2和es2），同样低C18:0含量对高C18:0含量呈部分显性。根据这些结果，各亲本推测的基因型（C18:0含量）如下：CAS - 3（25.0% C18:0）=es1es1es2es2；RDF - 1 - 532（8.0% C18:0）=Es1Es1es2es2；HA - 89（4.6% C18:0）=Es1Es1Es2Es2。推测的基因型与其C18:0含量之间的关系表明，Es1基因座对C18:0含量的影响大于Es2基因座。显然，诱变处理导致RDF - 1 - 532中的Es1突变为es1。
