Velasco L, Nabloussi A, De Haro A, Fernández-Martínez J M
Instituto de Agricultura Sostenible (CSIC), Apartado 4084, E-14080 Córdoba, Spain.
Theor Appl Genet. 2003 Sep;107(5):823-30. doi: 10.1007/s00122-003-1295-z. Epub 2003 May 17.
Seed oil of current zero erucic-acid germplasm of Ethiopian mustard ( Brassica carinata A. Braun) is characterized by a low concentration of oleic acid and high concentrations of linoleic and linolenic acids. Sources of increased oleic-acid (HO) and reduced linolenic-acid (LL) concentration have been developed separately in high erucic-acid germplasm. The objectives of the present research were to study the inheritance of the HO and LL traits in crosses HO x LL, and to develop HOLL recombinants, both in high erucic-acid and zero erucic-acid backgrounds. The HO mutant N2-3591 (about 20% oleic acid compared to 9% in conventional high erucic-acid materials), was reciprocally crossed with the LL lines N2-4961 and HF-186 (both with about 5% linolenic acid compared to 12% in standard high erucic-acid materials). Increased oleic acid concentration of N2-3591 was found to be controlled by alleles at one locus (Ol), whereas three different loci for reduced linolenic-acid concentration (Ln, Ln1 and Ln2) were identified in N2-4961 and HF-186. Crosses between N2-3591 and N2-4961 generated HOLL recombinants where levels of increased oleic-acid and reduced linolenic-acid were similar to those of the parents. However, a transgressive segregation for oleic acid was observed in crosses between N2-3591 and HF-186, where F(2) seeds with up to 29.7% oleic acid were obtained, in comparison to an upper limit of 25.1% in the N2-3591 parent grown in the same environment. The transgressive increased oleic-acid was expressed in the F(3) generation and was attributed to the presence of a second locus, designated Ol2. The transgressive trait was transferred to the zero erucic-acid line 25X-1, resulting in a zero erucic-acid germplasm with very high oleic-acid concentration (83.9% compared to 32.9% in 25X-1) and low linolenic-acid concentration (5.0% compared to 16% in 25X-1). Additionally, two other lines exhibiting different stable levels of increased oleic-acid (70.7% and 79.5%, respectively) and reduced levels of linolenic-acid (7.5% and 8.7%, respectively) were isolated.
埃塞俄比亚芥（Brassica carinata A. Braun）当前零芥酸种质的种子油具有油酸浓度低、亚油酸和亚麻酸浓度高的特点。在高芥酸种质中已分别培育出油酸含量增加（HO）和亚麻酸含量降低（LL）的种质。本研究的目的是研究HO×LL杂交组合中HO和LL性状的遗传，并在高芥酸和零芥酸背景下培育HOLL重组体。HO突变体N2 - 3591（油酸含量约为20%，而传统高芥酸材料中为9%）与LL品系N2 - 4961和HF - 186进行正反交（这两个品系的亚麻酸含量均约为5%，而标准高芥酸材料中为12%）。发现N2 - 3591中增加的油酸浓度受一个位点（Ol）的等位基因控制，而在N2 - 4961和HF - 186中鉴定出三个不同的降低亚麻酸浓度的位点（Ln、Ln1和Ln2）。N2 - 3591与N2 - 4961杂交产生了HOLL重组体，其油酸增加和亚麻酸降低的水平与亲本相似。然而，在N2 - 3591与HF - 186的杂交中观察到油酸的超亲分离，在相同环境下种植的N2 - 3591亲本中油酸上限为25.1%，而其F(2)种子中油酸含量高达29.7%。超亲增加的油酸在F(3)代中表现出来，这归因于第二个位点Ol2的存在。该超亲性状被转移到零芥酸品系25X - 1中，从而得到了一种零芥酸种质，其油酸浓度非常高（83.9%，而25X - 1中为32.9%）且亚麻酸浓度低（5.0%，而25X - 1中为16%）。此外，还分离出另外两个品系，它们分别表现出不同的稳定增加的油酸水平（分别为70.7%和79.5%）和降低的亚麻酸水平（分别为7.5%和8.7%）。
