del Río M, de Haro A, Fernández-Martínez J M
Instituto de Agricultura Sostenible (CSIC), Apartado 4084, E-14080 Córdoba, Spain.
Theor Appl Genet. 2003 Aug;107(4):643-51. doi: 10.1007/s00122-003-1293-1. Epub 2003 May 21.
Two Ethiopian mustard ( Brassica carinata A. Braun) lines with low (about 10%) and zero erucic acid (C22:1) have been obtained. The low C22:1 mutant line L-2890 was isolated after a chemical-mutagen treatment of C-101 seeds (about 40% C22:1). The zero C22:1 line L-25X-1 was obtained by interspecific crossing. Our objective was to determine the genetic control of low and zero C22:1 contents in these lines and the relationship between the loci controlling these traits. Reciprocal crosses between L-2890, L-25X-1 and high C22:1 lines, and between L-2890 and L-25X-1, were made. The F(1), F(2) and BC(1) F(1) generations were obtained. No maternal or cytoplasmic effects for C22:1 content were observed in any of the crosses. The analysis of the fatty acid composition in the segregating populations from the crosses of L-2890 with the high C22:1 lines C-101 and L-1630 indicated that the segregation patterns fitted a model of two alleles at two loci, M1 and M2, with partial (near complete) dominance for high concentration. The segregation patterns in the cross of the zero C22:1 line L-25X-1 with the high C22:1 line L-1630, were explained on the basis of two genes, E1 and E2, with additive gene action. The F(1) and segregating generations of the crosses L-2890 x L-25X-1 showed a strong transgressive segregation with C22:1 values of up to 50.0%, four-fold higher than those of L-2890. The analyses of the F(2), BC(1)F(1) and F(3) generations indicated that the combination of alleles at four loci, M(1) and M(2) in L-2890 and E(1) and E(2) in L-25X-1, controlled the transgressive segregation for C22:1. The proposed genotypes (C22:1 content) for each parent were as follows: L-2890 (10% C22:1) = m(1) m(1) m(2) m(2) E(1) E(1) E(2) E(2); L-25X-1 (0% C22:1) = M(1) M(1) M(2) M(2) e(1) e(1) e(2) e(2); and C-101 (45% C22:1) = M(1) M(1) M(2) M(2) E(1) E(1) E(2) E(2).
已获得两个芥酸（C22:1）含量低（约10%）和零芥酸的埃塞俄比亚芥（Brassica carinatainata A. Braun）品系。低C22:1突变体系L - 2890是在对C - 101种子（约40% C22:1）进行化学诱变处理后分离得到的。零C22:1品系L - 25X - 1是通过种间杂交获得的。我们的目标是确定这些品系中低C22:1和零C22:1含量的遗传控制以及控制这些性状的基因座之间的关系。进行了L - 2890、L - 25X - 1与高C22:1品系之间以及L - 2890与L - 25X - 1之间的正反交。获得了F(1)、F(2)和BC(1)F(1)代。在任何杂交组合中均未观察到C22:1含量的母本或细胞质效应。对L - 2890与高C22:1品系C - 101和L - 1630杂交的分离群体中脂肪酸组成的分析表明，分离模式符合两个基因座（M1和M2）上两个等位基因的模型，高浓度表现为部分（接近完全）显性。零C22:1品系L - 25X - 1与高C22:1品系L - 1630杂交的分离模式基于两个基因（E1和E2）的加性基因作用来解释。L - 2890×L - 25X - 1杂交的F(1)代和分离世代表现出强烈的超亲分离，C22:1值高达50.0%，比L - 2890高四倍。对F(2)、BC(1)F(1)和F(3)代的分析表明，L - 2890中的等位基因M(1)和M(2)以及L - 25X - 1中的等位基因E(1)和E(2)在四个基因座上的组合控制了C22:1的超亲分离。每个亲本的推测基因型（C22:1含量）如下：L - 2890（10% C22:）= m(1) m(1) m(2) m(2) E(1) E(1) E(2) E(2)；L - 25X - 1（0% C22:1）= M(1) M(1) M(2) M(2) e(1) e(1) e(2) e(2)；C - 101（45% C22:1）= M(1) M(1) M(2) M(2) E(1) E(1) E(2) E(2)。
