Rabilloud Thierry
CEA-DSV-iRTSV/CBM and UMR CNRS-UJF 5249, CEA Grenoble, Grenoble, France.
Methods Mol Biol. 2012;893:61-73. doi: 10.1007/978-1-61779-885-6_5.
Silver staining is used to detect proteins after electrophoretic separation on polyacrylamide gels. It -combines excellent sensitivity (in the low nanogram range) with the use of very simple and cheap equipment and chemicals. For its use in proteomics, two important additional features must be considered, compatibility with mass spectrometry and quantitative response. Both features are discussed in this chapter, and optimized silver staining protocols are proposed.
银染法用于在聚丙烯酰胺凝胶上进行电泳分离后检测蛋白质。它将出色的灵敏度（低纳克范围）与使用非常简单且廉价的设备和化学试剂相结合。对于其在蛋白质组学中的应用，必须考虑两个重要的附加特性，即与质谱的兼容性和定量响应。本章将讨论这两个特性，并提出优化的银染方案。
