年龄相关性黄斑变性患者血浆蛋白的比较蛋白质组学分析
Comparative proteomic analysis of plasma proteins in patients with age-related macular degeneration.
作者信息
Xu Xin-Rong, Zhong Lu, Huang Bing-Lin, Wei Yuan-Hua, Zhou Xin, Wang Ling, Wang Fu-Qiang
机构信息
Department of Ophthalmology, Jiangsu Province Hospital of Traditional Chinese Medicine, Nanjing 210029, Jiangsu Province, China.
Department of Clinical Laboratory, Jiangsu Province Hospital of Traditional Chinese Medicine, Nanjing 210029, Jiangsu Province, China.
出版信息
Int J Ophthalmol. 2014 Apr 18;7(2):256-63. doi: 10.3980/j.issn.2222-3959.2014.02.12. eCollection 2014.
AIM
To find the significant altered proteins in age-related macular degeneration (AMD) patients as potential biomarkers of AMD.
METHODS
A comparative analysis of the protein pattern of AMD patients versus healthy controls was performed by means of proteomic analysis using two-dimensional gel electrophoresis followed by protein identification with MALDI TOF/TOF mass spectrometry.
RESULTS
We identified 28 proteins that were significantly altered with clinical relevance in AMD patients. These proteins were involved in a wide range of biological functions including immune responses, growth cytokines, cell fate determination, wound healing, metabolism, and anti-oxidance.
CONCLUSION
These results demonstrate the capacity of proteomic analysis of AMD patient plasma. In addition to the utility of this approach for biomarker discovery, identification of alterations in endogenous proteins in the plasma of AMD patient could improve our understanding of the disease pathogenesis.
目的
寻找年龄相关性黄斑变性（AMD）患者中显著改变的蛋白质，作为AMD的潜在生物标志物。
方法
采用二维凝胶电泳进行蛋白质组分析，随后用基质辅助激光解吸电离飞行时间/飞行时间质谱进行蛋白质鉴定，对AMD患者与健康对照的蛋白质图谱进行比较分析。
结果
我们鉴定出28种在AMD患者中具有临床相关性的显著改变的蛋白质。这些蛋白质参与了广泛的生物学功能，包括免疫反应、生长细胞因子、细胞命运决定、伤口愈合、代谢和抗氧化。
结论
这些结果证明了对AMD患者血浆进行蛋白质组分析的能力。除了这种方法在生物标志物发现方面的效用外，鉴定AMD患者血浆中内源性蛋白质的改变有助于我们更好地理解疾病发病机制。
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