二维电泳凝胶的银染。

Silver Staining of 2D Electrophoresis Gels.

机构信息

Chemistry and Biology of Metals, Univ. Grenoble Alpes, CNRS UMR5249, IRIG-DIESE-CBM, CEA Grenoble, Grenoble Cedex 9, France.

出版信息

Methods Mol Biol. 2021;2228:63-75. doi: 10.1007/978-1-0716-1024-4_6.

DOI:10.1007/978-1-0716-1024-4_6

PMID:33950484

Abstract

Silver staining is used to detect proteins after electrophoretic separation on polyacrylamide gels. It combines excellent sensitivity (in the low nanogram range) with the use of very simple and cheap equipment and chemicals. For its use in proteomics, two important additional features must be considered, compatibility with mass spectrometry and quantitative response. Both features are discussed in this chapter, and optimized silver staining protocols are proposed.

摘要

银染技术用于检测聚丙烯酰胺凝胶电泳分离后的蛋白质。它结合了优异的灵敏度（可达纳克级）、简单廉价的设备和试剂。在蛋白质组学研究中，还需要考虑两个重要的附加特性，即与质谱技术的兼容性和定量响应。本章将讨论这两个特性，并提出优化的银染方案。