Randjelovic Pavle, Veljkovic Slavimir, Stojiljkovic Nenad, Jankovic-Velickovic Ljubinka, Sokolovic Dusan, Stoiljkovic Milan, Ilic Ivan
Department of Physiology, Faculty of Medicine, University of Nis, 18000 Nis, Serbia.
ScientificWorldJournal. 2012;2012:390613. doi: 10.1100/2012/390613. Epub 2012 Apr 30.
Gentamicin (GM) is a widely used antibiotic against serious and life-threatening infections, but its usefulness is limited by the development of nephrotoxicity. The present study was designed to determine the protective effect of salicylic acid (SA) in gentamicin-induced nephrotoxicity in rats. Quantitative evaluation of gentamicin-induced structural alterations and degree of functional alterations in the kidneys were performed by histopathological and biochemical analyses in order to determine potential beneficial effects of SA coadministration with gentamicin. Gentamicin was observed to cause a severe nephrotoxicity which was evidenced by an elevation of serum urea and creatinine levels. The significant increases in malondialdehyde (MDA) levels and protein carbonyl groups indicated that GM-induced tissue injury was mediated through oxidative reactions. On the other hand, simultaneous SA administration protected kidney tissue against the oxidative damage and the nephrotoxic effect caused by GM treatment. Exposure to GM caused necrosis of tubular epithelial cells. Necrosis of tubules was found to be prevented by SA pretreatment. The results from our study indicate that SA supplement attenuates oxidative-stress associated renal injury by reducing oxygen free radicals and lipid peroxidation in gentamicin-treated rats.
庆大霉素（GM）是一种广泛用于治疗严重及危及生命感染的抗生素，但其效用因肾毒性的出现而受到限制。本研究旨在确定水杨酸（SA）对庆大霉素诱导的大鼠肾毒性的保护作用。通过组织病理学和生化分析对庆大霉素诱导的肾脏结构改变和功能改变程度进行定量评估，以确定SA与庆大霉素联合给药的潜在有益效果。观察到庆大霉素会导致严重的肾毒性，血清尿素和肌酐水平升高即为证据。丙二醛（MDA）水平和蛋白质羰基显著增加表明，GM诱导的组织损伤是通过氧化反应介导的。另一方面，同时给予SA可保护肾脏组织免受GM治疗引起的氧化损伤和肾毒性作用。接触GM会导致肾小管上皮细胞坏死。发现SA预处理可预防肾小管坏死。我们的研究结果表明，SA补充剂通过减少庆大霉素处理大鼠体内的氧自由基和脂质过氧化，减轻了与氧化应激相关的肾损伤。
