School of Medicine, University of Colima, Colima 28040, Mexico.
Department of Research, Esteripharma SA de CV, Atlacomulco 50450, Mexico.
Medicina (Kaunas). 2023 Feb 17;59(2):397. doi: 10.3390/medicina59020397.
: Gentamicin (GM) is a nephrotoxic aminoglycoside. Neutral electrolyzed saline (SES) is a compound with anti-inflammatory, antioxidant, and immunomodulatory properties. The objective of the present study was to evaluate whether kidney damage by GM can be prevented and/or reversed through the administration of SES. : The study was carried out as a prospective, single-blind, five-arm, parallel-group, randomized, preclinical trial. The nephrotoxicity model was established in male BALB/c mice by administering GM at a dose of 100 mg/kg/day intraperitoneally for 30 days, concomitantly administering (+) SES or placebo (physiologic saline solution), and then administering SES for another 30 days after the initial 30 days of GM plus SES or placebo. At the end of the test, the mice were euthanized, and renal tissues were evaluated histopathologically. : The GM + placebo group showed significant tubular injury, interstitial fibrosis, and increased interstitial infiltrate of inflammatory cells compared with the group without GM. Tubular injury and interstitial fibrosis were lower in the groups that received concomitant GM + SES compared with the GM + placebo group. SES administration for 30 days after the GM administration periods (GM + placebo and GM + SES for 30 days) did not reduce nephrotoxicity. : Intraperitoneal administration of SES prevents gentamicin-induced histologic nephrotoxicity when administered concomitantly, but it cannot reverse the damage when administered later.
庆大霉素（GM）是一种肾毒性氨基糖苷类药物。中性电解盐水（SES）是一种具有抗炎、抗氧化和免疫调节特性的化合物。本研究的目的是评估 SES 的给药是否可以预防和/或逆转 GM 引起的肾脏损伤。
这项研究是一项前瞻性、单盲、五臂、平行组、随机、临床前试验。通过腹腔内给予 100mg/kg/天 GM 30 天，建立雄性 BALB/c 小鼠的肾毒性模型，同时给予（+）SES 或安慰剂（生理盐水），然后在 GM 加 SES 或安慰剂最初 30 天后再给予 SES 30 天。在测试结束时，处死小鼠并对肾脏组织进行组织病理学评估。
GM + 安慰剂组与未给予 GM 的组相比，表现出明显的肾小管损伤、间质纤维化和间质炎症细胞浸润增加。与 GM + 安慰剂组相比，同时给予 GM + SES 的组肾小管损伤和间质纤维化程度较低。GM 给药后 30 天（GM + 安慰剂和 GM + SES 各 30 天）给予 SES 给药并未减轻肾毒性。
腹腔内给予 SES 可预防同时给予 GM 引起的组织学肾毒性，但不能逆转后期给予 GM 引起的损伤。
