Ohtani S, Yamamoto K
Department of Forensic Medicine, Kanagawa Dental College, Japan.
Nihon Hoigaku Zasshi. 1990 Aug;44(4):346-51.
To investigate the racemization of dentinal amino acids furthermore, and thereby to estimate age more accurately, fundamental heating experiments dealing with aspartic acid in dentine were carried out. The racemization velocity was determined and compared not only for the total amino acids (TAA), but for insoluble collagen (IC) as well as soluble peptide (SP) fractions. In TAA and IC, with the passage of time, their racemization deviated from the mode of the primary reaction, whereas in the SP, an extremely stable increase in D/L ratio was maintained for a longer time, fulfilling the mode of the primary reaction. When samples are left drying, the racemization velocity was greatest in the IC but considerably less in the SP, indicating results opposite to those found in the living state.
为了进一步研究牙本质氨基酸的消旋作用，从而更准确地估计年龄，开展了关于牙本质中天冬氨酸的基础加热实验。不仅测定并比较了总氨基酸（TAA）的消旋速度，还测定并比较了不溶性胶原蛋白（IC）以及可溶性肽（SP）组分的消旋速度。在TAA和IC中，随着时间的推移，它们的消旋作用偏离了一级反应模式，而在SP中，D/L比值保持了极长时间的极其稳定的增加，符合一级反应模式。当样本处于干燥状态时，IC中的消旋速度最快，而SP中的消旋速度则要小得多，这表明与在活体状态下发现的结果相反。
