Ohtani S, Yamamoto K
Department of Forensic Medicine, Kanagawa Dental College, Japan.
Nihon Hoigaku Zasshi. 1990 Aug;44(4):342-5.
We studied the possibility of estimating age through the amino acid racemization of acid-soluble dentinal peptide (SP). The correlation of the D/L ratio of aspartic acid and the actual age was the highest in the SP extracted with 1 M HCl (r = 0.996, sigma = +/- 0.8), followed by the total amino acids (TAA:r = 0.995, sigma = +/- 1.0), and the SP extracted with 2 M HCl (r = 0.980, sigma = +/- 1.9) and then SP extracted with 5 M HCl (r = 0.951, sigma = +/- 2.7). The rate of the racemization reaction decreased in the order of SP extracted with 1 M HCl, 2 M HCl, 5 M HCl and the total amino acids (TAA). Consequently, an extraction with 1 M HCl seemed best for estimating the age from SP to serve actual cases.
我们研究了通过酸溶性牙本质肽（SP）的氨基酸消旋化来估计年龄的可能性。用1M盐酸提取的SP中天冬氨酸的D/L比值与实际年龄的相关性最高（r = 0.996，标准差=±0.8），其次是总氨基酸（TAA：r = 0.995，标准差=±1.0），用2M盐酸提取的SP（r = 0.980，标准差=±1.9），然后是用5M盐酸提取的SP（r = 0.951，标准差=±2.7）。消旋化反应速率按1M盐酸提取的SP、2M盐酸提取的SP、5M盐酸提取的SP和总氨基酸（TAA）的顺序降低。因此，用1M盐酸提取似乎最适合从SP估计年龄以用于实际案例。
