Akpor Oghenerobor B, Akinwusi Oluwafunto D, Ogunnusi Tolulope A
Department of Biological Sciences, Afe Babalola University, PMB 5454, Ado-Ekiti, Ekiti State, Nigeria.
Department of Microbiology and Food Science, Landmark University, PMB 1001, Omu-Aran, Kwara State, Nigeria.
Heliyon. 2021 Nov 19;7(11):e08447. doi: 10.1016/j.heliyon.2021.e08447. eCollection 2021 Nov.
The use of biopesticides in pest management and pre-harvest disease and crop pest control have been advocated in recent years. This is because of their eco-friendliness and suitability for pest control in the agricultural industry. The objective of this study was to determine the antibacterial and pesticidal potential against sugar ant of metabolites produced by species. The species were and Metabolite production was carried out in batch experimental setup. The inoculated flasks were incubated in an incubator shaker for 120 h at temperature of 37 °C ± 2 °C. Metabolite extraction was carried out using the acid precipitation method. The crude metabolites were characterized using Fourier Transform Infrared (FTIR) and Gas Chromatography Mass Spectroscopy (GC-MS). Antibacterial activity of the metabolites was carried out both in agar and broth media while pesticidal potential was carried out using the diet-fed approach. All the metabolites showed antibacterial activity against the test pathogens used for investigation. This was irrespective of whether they were used singly or in combination. Generally, the rate of kill of the sugar ants by the respective metabolites was directly proportional to metabolite concentration in the diet. In the control diet setup with no added metabolite, no mortality was recorded throughout the period of incubation. The study findings gave an indication of the potential of these metabolites for possible control of phytopathogens.
近年来，生物农药在害虫管理以及收获前的病害和农作物害虫防治中的应用得到了提倡。这是因为它们具有生态友好性且适用于农业产业中的害虫防治。本研究的目的是确定[物种名称]产生的代谢产物对糖蚁的抗菌和杀虫潜力。这些物种是[具体物种名称1]和[具体物种名称2]。代谢产物的生产在分批实验装置中进行。接种后的烧瓶在恒温振荡器中于37℃±2℃下孵育120小时。使用酸沉淀法进行代谢产物提取。粗代谢产物通过傅里叶变换红外光谱（FTIR）和气相色谱 - 质谱联用（GC - MS）进行表征。代谢产物的抗菌活性在琼脂和肉汤培养基中进行，而杀虫潜力则采用喂食法进行测定。所有代谢产物对用于研究的测试病原体均表现出抗菌活性。无论它们是单独使用还是组合使用都是如此。一般来说，各代谢产物对糖蚁的杀灭率与饲料中代谢产物的浓度成正比。在未添加代谢产物的对照饲料设置中，在整个孵育期间均未记录到死亡情况。研究结果表明了这些代谢产物在控制植物病原体方面的潜力。
