Han Bingbing, Wang Shijun, Zhang Fayan, Zhao Haijun, Wang Chenggang
College of Basic Medical Sciences, Shandong University of Traditional Chinese Medicine, Jinan 250355, China.
Zhongguo Zhong Yao Za Zhi. 2012 Feb;37(4):500-4.
To study the influence of Aconiti Lateralis Radix Praeparata on asthenia cold syndrome rats with whole genome gene expression of liver by gene chip technique.
The asthenia cold syndrome rat models were established by administering traditional Chinese medicine raw Gypsum Fibrosum, Gentianae Radix, Phellodendri Chinensis Cortex and Anemarrhenae Rhizoma. After treated with Aconiti Lateralis Radix Praeparata, the rats' liver gene expressions were detected using gene chip. Differential expression genes were screened for gene function annotation, and some genes were selected to check the accuracy of the results by RT-PCR.
Compared with the asthenia cold model group, the asthenia cold treatment group showed 212 differential expression genes, mainly involving function of immune response and oxidoreductase activity.
Aconiti Lateralis Radix Praeparata is proved to have an effect on up-regulating immune response-related genes and oxidizing oxidoreductase activity-related genes of asthenia cold syndrome rats and may be a molecular mechanism for classical warm-nature medicine Aconiti Lateralis Radix Praeparata in warming meridians and dissipating cold.
采用基因芯片技术研究制附子对虚寒证大鼠肝脏全基因组基因表达的影响。
运用中药生石膏、龙胆、黄柏、知母造虚寒证大鼠模型。制附子处理后，采用基因芯片检测大鼠肝脏基因表达情况。对差异表达基因进行基因功能注释筛选，并选取部分基因通过RT-PCR验证结果准确性。
与虚寒模型组相比，虚寒治疗组有212个差异表达基因，主要涉及免疫应答功能和氧化还原酶活性。
证明制附子对虚寒证大鼠免疫应答相关基因及氧化还原酶活性相关基因有上调作用，可能是经典温热药制附子温经散寒的分子机制。
