Sun Ting, Du Gang-Jun, Zhang Ya-Ping, Li Jia-Huan, Liu Wei-Jie, Wang Ying-Ying
Drug Research Institute, Pharmaceutical College of Henan University, Kaifeng 475001, China.
Zhongguo Zhong Yao Za Zhi. 2012 Oct;37(20):3097-101.
To study Chinese medicine (CM) signs and symptoms of urethane-induced lung cancer in mice, and observe the effect of Aconiti Lateralis Radix Praeparata and Taraxaci Herba on symptoms in mice and tumor progress.
The mice were intraperitoneally injected with urethane twice a week for consecutively five weeks to establish a lung cancer model. The changes in their appearance, body temperature and auricle microcirculation were observed in carcinogenic process. CM signs and symptoms of urethane-induced lung cancer in mice were evaluated with energy metabolism, erythrocytic ATP emzymatic activity and hemorrheological index. During the tumor model was induced, Aconiti Lateralis Radix Praeparata and Taraxaci Herba were used to treat the mice and observe their effect on symptoms in mice and tumor progress.
During urethane was used to induce lung cancer, the mice had gradually become chill, lazy, hunched, with reduction in temperature, cyanosis in auricle and tail. Meanwhile, their energy metabolism and erythrocytic ATP enzymatic activity reduced, whereas their whole blood viscosity and erythrocytic aggregate index increased. Taraxaci Herba showed an effect on enhancing above symptoms and signs but had no effect on tumor progress. Aconiti Lateralis Radix Praeparata showed an effect on reducing above symptoms and signs and preventing tumor progress.
Mice with urethane-induced lung cancer show CM signs and symptoms of congealing cold with blood stasis. The treatment with Aconiti Lateralis Radix Praeparata can alleviate symptoms and signs in mice and prevent tumor progress.
研究乌拉坦诱导的小鼠肺癌的中医症状体征，观察附子与蒲公英对小鼠症状及肿瘤进展的影响。
小鼠每周腹腔注射乌拉坦2次，连续5周以建立肺癌模型。在致癌过程中观察小鼠外观、体温及耳廓微循环的变化。用能量代谢、红细胞ATP酶活性及血液流变学指标评估乌拉坦诱导的小鼠肺癌的中医症状体征。在诱导肿瘤模型期间，用附子与蒲公英治疗小鼠，观察其对小鼠症状及肿瘤进展的影响。
在使用乌拉坦诱导肺癌期间，小鼠逐渐变得畏寒、慵懒、弓背，体温降低，耳廓及尾巴发绀。同时，其能量代谢及红细胞ATP酶活性降低，而全血黏度及红细胞聚集指数升高。蒲公英对加重上述症状体征有作用，但对肿瘤进展无作用。附子对减轻上述症状体征及阻止肿瘤进展有作用。
乌拉坦诱导的肺癌小鼠表现出寒凝血瘀的中医症状体征。用附子治疗可减轻小鼠症状体征并阻止肿瘤进展。
