Division of Biology 156-29, California Institute of Technology, 1200 East California Boulevard, Pasadena, California 91125, United States.
Nano Lett. 2012 Jul 11;12(7):3690-4. doi: 10.1021/nl301480h. Epub 2012 Jun 8.
We exploit the optical and spatial features of subwavelength nanostructures to examine individual receptors on the plasma membrane of living cells. Receptors were sequestered in portions of the membrane projected into zero-mode waveguides. Using single-step photobleaching of green fluorescent protein incorporated into individual subunits, the resulting spatial isolation was used to measure subunit stoichiometry in α4β4 and α4β2 nicotinic acetylcholine and P2X2 ATP receptors. We also show that nicotine and cytisine have differential effects on α4β2 stoichiometry.
我们利用亚波长纳米结构的光学和空间特征来检测活细胞膜上的单个受体。受体被隔离在零模波导中突出的膜部分内。通过将绿色荧光蛋白掺入到单个亚基中单步光漂白,利用所得的空间隔离来测量α4β4 和 α4β2 烟碱型乙酰胆碱和 P2X2 ATP 受体中亚基的计量比。我们还表明,尼古丁和烟碱具有不同的α4β2 计量比效应。