Math1基因转移诱导大鼠前庭感觉上皮新霉素耳毒性后I型毛细胞再生。
Type I hair cell regeneration induced by Math1 gene transfer following neomycin ototoxicity in rat vestibular sensory epithelium.
作者信息
Xu Jin-Cao, Huang De-liang, Hou Zhao-Hui, Guo Wei-Wei, Sun Jian-He, Zhao Li-Dong, Yu Ning, Young Wie-Yen, He David Zhi-Zhou, Yang Shi-Ming
机构信息
Department of Otolaryngology Head and Neck Surgery, Institute of Otolaryngology, Chinese PLA General Hospital, Beijing, China.
出版信息
Acta Otolaryngol. 2012 Aug;132(8):819-28. doi: 10.3109/00016489.2012.673233. Epub 2012 Jun 5.
CONCLUSION
In the current study, hair cells of vestibular terminal organs in rats were completely eliminated with trans-scala vestibuli injection of neomycin, and then the Math1 gene was transferred. It was shown that type I vestibular hair cells were regenerated and synapses were formed.
OBJECTIVES
The objective of this study was to identify the cell type of the regenerated vestibular hair cells and relative innervation and synaptic linkage after hair cells of vestibular terminal organs in rats were completely eliminated.
METHODS
Neomycin injection was used to eliminate all the vestibular terminal organs, and then the animals were treated with an injection of Ad-Math1-EGFP in the scala vestibuli of the cochlea.
RESULTS
Math1 gene transfer into the inner ear induced type I hair cell regeneration and synaptic formation. However, neither the number nor the appearance of the hair cells was normal.
结论
在本研究中，通过经前庭阶注射新霉素完全消除大鼠前庭终器的毛细胞，然后转入Math1基因。结果显示I型前庭毛细胞再生并形成了突触。
目的
本研究的目的是在大鼠前庭终器毛细胞被完全消除后，鉴定再生的前庭毛细胞的细胞类型以及相关的神经支配和突触连接。
方法
采用注射新霉素的方法消除所有前庭终器，然后在耳蜗的前庭阶注射Ad-Math1-EGFP对动物进行处理。
结果
Math1基因转入内耳诱导了I型毛细胞再生和突触形成。然而，毛细胞的数量和外观均不正常。
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